| 鄂西地区青蒿遗传多样性研究 |
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| 引用本文： | 石开明,艾训儒,丁莉,周毅峰.鄂西地区青蒿遗传多样性研究[J].湖北农业科学,2008,47(7). |
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| 作者姓名： | 石开明 艾训儒 丁莉 周毅峰 |
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| 作者单位： | 1. 湖北民族学院,生物科学与技术学院,湖北,恩施,445000;湖北民族学院,生物资源保护与利用湖北省重点实验室,湖北,恩施,445000;湖北大学中药生物技术湖北省重点实验室,武汉,430062
2. 湖北民族学院,生物科学与技术学院,湖北,恩施,445000; |
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| 基金项目： | 中药生物技术湖北省重点实验室开放基金项目
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生物资源保护与利用湖北省重点实验室项目 |
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| 摘 要： | 利用RAPD和ISSR分子标记技术对11份鄂西野生青蒿种质进行了遗传多样性研究.结果表明,筛选出的8条RAPD和8条ISSR引物分别产生86条和102条扩增带,其中多态性条带分别为53条和62条,分别占总数的61.63%和60.78%.用POPGENE对两种标记方法的遗传多样性进行计算.有效等位基因数分别为1.355 2和1.370 7,基因多样性为0.2110和0.214 3,Shannon信息指数为0.318 8和0.321 6.以Nei氏遗传距离矩阵按UPGMA方法聚类分析RAPD和ISSR标记的遗传距离分别为0.035 5～0.411 3和0.142 3～0.401 1,平均值各为0.228 8和0.233 8,表明青蒿种质具有比较丰富的遗传变异,同时也说明,尽管Mantel相关性检测两种标记方法相关性较低(r=0.543 9,P0..05).但都可以有效地揭示青蒿种质的遗传多样性状况.
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| 关 键 词： | 青蒿 遗传多样性 RAPD分子标记 ISSR分子标记 |
Study on Genetic Diversity of Artemisiae annie Varieties in the Westen Hubei |
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| SHI Kai-ming,AI Xun-ru,Ding Li,ZHOU Yi-feng.Study on Genetic Diversity of Artemisiae annie Varieties in the Westen Hubei[J].Hubei Agricultural Sciences,2008,47(7). |
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| Authors: | SHI Kai-ming AI Xun-ru Ding Li ZHOU Yi-feng |
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| Institution: | SHI Kai-ming1ab,2,AI Xun-ru1a,Ding Li1a,ZHOU Yi-feng1ab,2(1a.Biological Science , Technical Department,1b.Key Laboratory for Protection , Utilization of Biological Resources of Hubei Province,1.Hubei Institute for Nationalities,Enshi 445000,Hubei,China,2.Key Laboratory for Biological Technology of Chinese Traditional Medicine of Hubei Province,Hubei University,Wuhan 430062,China) |
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| Abstract: | The genetic diversity of 11 Artemisiae annie types in the Westen Hubei have been investigated with the techniques of RAPD and ISSR.The results showed that 86 and 102 bands were obtained by RAPD and ISSR markers amplified through 8 selected primers respectively.53 bands and 62 bands were polymorphic,which accounted for 61.63% and 60.78% respectively.Through POPGENE analysis,effective number of alleles per locus,gene diversity and Shannon's information index were 1.355 2,0.211 0 and 0.318 8 by RAPD,and were 1... |
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| Keywords: | Artemisiae annie genetic diversity RAPD molecular marker ISSR molecular marker |
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