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| 绒山羊胚胎期和兴盛期皮肤毛囊差异表达基因研究 | |
| 引用本文： | 李玉荣,尹俊,张燕军,刘羿羿,李金泉.绒山羊胚胎期和兴盛期皮肤毛囊差异表达基因研究[J].湖北农业科学,2008,47(2):146-148. |
| 作者姓名： | 李玉荣 尹俊 张燕军 刘羿羿 李金泉 |
| 作者单位： | 1. 内蒙古农业大学,动物科学与医学学院,呼和浩特 010018;内蒙古农牧业科学院畜牧研究所,呼和浩特 010018 2. 内蒙古农业大学,生物工程学院,呼和浩特 010018 3. 内蒙古农业大学,动物科学与医学学院,呼和浩特 010018 |
| 摘 要： | 应用抑制性消减杂交技术成功构建了内蒙古绒山羊胚胎期和兴盛期皮肤毛囊的消减cDNA文库.从两个文库中分别随机挑取50个克隆测序分析,测序结果经Genbank同源性比对.表明在胚胎期消减cDNA文库中得到15个特异表达基因片段,包含1个未知新序列;在兴盛期消减cDNA文库中得到19个特异表达基因片段.包含2个未知新序列. |
| 关 键 词： | 抑制性消减杂交 绒山羊 胚胎期 兴盛期 消减cDNA文库 |
| 文章编号： | 0439-8114（2008）02-0146-03 |
| 收稿时间： | 2007-10-08 |
| 修稿时间： | 2007年10月8日 |
Study on Differently Expressed Genes in Skin Follicle of Cashmere Goat at the Embryonic Stage and Anagen Stage |
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| LI Yu-rong,YIN Jun,ZHANG Yan-jun,LIU Yi-yi,LI Jin-quan.Study on Differently Expressed Genes in Skin Follicle of Cashmere Goat at the Embryonic Stage and Anagen Stage[J].Hubei Agricultural Sciences,2008,47(2):146-148. | |
| Authors: | LI Yu-rong YIN Jun ZHANG Yan-jun LIU Yi-yi LI Jin-quan |
| Institution: | LI Yu-rong1a,2,YIN Jun1b,ZHANG Yan-jun1a,LIU Yi-yi1a,LI Jin-quan1a (1.Inner Mongolia Agricultural University a.College of Animal Science , Medical Science,b.Institute of Bioengineering,Huhehaote 010018,China,2.Institute of Animal Sciences,Inner Mongolia Academy of Angriculture , Animal Husb,ry Sciences,China) |
| Abstract: | The skin follicle subtractive cDNA library of the Inner Mongolian cashmere goat was constructed by suppression subtractive hybridization. 50 clones which were derived from the embryonic stage and anagen eDNA library respectively were sequenced and blasted with known gene in Genbank. 15 cDNA fragments were obtained from the embryonic stage eDNA library, 14 fragments corresponded to known gene and one was unknown sequence. 19 eDNA fragments were obtained from anagen eDNA library,17 cDNA fragments corresponded to known gene and two was unknown sequence . |
| Keywords: | suppression subtractive hybridization cashmere goat embryonic stage anagen subtractive eDNA library |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |