| PCR法构建小鼠α-1,3-GT基因沉默载体研究 |
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| 引用本文： | 刘中华,谷习文,郑新民,魏庆信,赵献军,肖红卫.PCR法构建小鼠α-1,3-GT基因沉默载体研究[J].湖北农业科学,2007,46(2):187-189. |
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| 作者姓名： | 刘中华 谷习文 郑新民 魏庆信 赵献军 肖红卫 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西,杨凌,712100
2. 湖北省农业科学院畜牧兽医研究所,武汉,430064 |
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| 基金项目： | 湖北省科技厅科研项目
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国家自然科学基金 |
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| 摘 要： | 在已报道的shRNA序列前加上人类H1启动子,设计一段沉默小鼠α-1,3-GT基因的siRNA序列,针对该序列分段设计引物,完全采用PCR法将其连接起来,然后把该序列克隆到pGEM-T载体中,构建出针对小鼠α-1,3-GT基因的特异性沉默载体,为进一步获得α-1,3-GT基因沉默小鼠,构建异种器官移植用小鼠模型奠定了基础.
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| 关 键 词： | 小干扰RNA 基因沉默 α-1 3-半乳糖苷转移酶 |
| 文章编号： | 0439-8114(2007)02-0187-03 |
| 修稿时间： | 10 13 2006 12:00AM |
Construction of Murine α-1,3-GT Gene Silencing Vector by PCR |
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| LIU Zhong-hua,GU Xi-wen,ZHENG Xin-min,WEI Qing-xin,ZHAO Xian-jun,XIAO Hong-wei.Construction of Murine α-1,3-GT Gene Silencing Vector by PCR[J].Hubei Agricultural Sciences,2007,46(2):187-189. |
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| Authors: | LIU Zhong-hua GU Xi-wen ZHENG Xin-min WEI Qing-xin ZHAO Xian-jun XIAO Hong-wei |
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| Abstract: | Through putting human H1 promoter before the shRNA reported,a siRNA sequence that would silence a-1,3-GT gene in murine was designed.a series of primer segments were subsequently devised and synthesized,and joined by PCR.A vector that could silence the murine a-1,3-GT gene was acquired by cloning the connected product into the pGEM-T vector.This would help us to constitute an important foundation for further development of a-1,3-GT knock down mice and for mouse model used for xenotransplantation research. |
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