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| 三株O_(78)、O_(137)混合血清型鸡大肠埃希菌iss基因的序列分析 | |
| 引用本文： | 樊琛,王宇,王会,王亚君,李一经.三株O_(78)、O_(137)混合血清型鸡大肠埃希菌iss基因的序列分析[J].湖北农业科学,2010,49(1). |
| 作者姓名： | 樊琛 王宇 王会 王亚君 李一经 |
| 作者单位： | 1. 东北农业大学动物医学院,哈尔滨,150030;聊城大学农学院,山东,聊城,252059 2. 山东省聊城市人民医院呼吸内科,山东,聊城,252000 3. 聊城大学农学院,山东,聊城,252059 4. 东北农业大学动物医学院,哈尔滨,150030;东北林业大学野生动物学院,哈尔滨,150040 5. 东北农业大学动物医学院,哈尔滨,150030 |
| 摘 要： | 采用1日龄雏鸡对3株O78、O137混合血清型鸡大肠埃希菌的致病性进行了测定,并扩增了iss基因。结果表明,3株O78、O137混合血清型鸡大肠埃希菌均对1日龄雏鸡具有较强的毒力。iss基因在3株O78、O137混合血清型鸡大肠埃希菌中的序列与已知的禽大肠埃希菌iss基因序列的同源性为100%,与人源大肠埃希菌iss基因序列的同源性为90.9%,显示了此基因的保守性。 |
| 关 键 词： | 鸡大肠埃希菌 iss基因 毒力 |
Sequnce Analysis of iss Gene in Three Avian Eschedchia coli Strains O78 and O137 |
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| FAN Chen,WANG YU,WANG Hui,WANG Ya-jun,LI Yi-jing.Sequnce Analysis of iss Gene in Three Avian Eschedchia coli Strains O78 and O137[J].Hubei Agricultural Sciences,2010,49(1). | |
| Authors: | FAN Chen WANG YU WANG Hui WANG Ya-jun LI Yi-jing |
| Abstract: | 3 avian Escherichia coli strains O_(78)/O_(137) were studied.The virulence of each avian E.coli strain O_(78)/O_(137) for 1-day-old chicks was detected,and each isolate was examined for the presence of iss gene using PCR techniques.Results showed that each avian E.coli strain O_(78)/O_(137) was strongly virulent.The sequence of iss from each avian E.coli strain O_(78)/O_(137) had 100%identity with AF042279 in GenBank and 90.9%identity with the iss gene from the human E.coli isolate.It suggested that iss gene might be highly conserved. |
| Keywords: | avian Escherichia coli iss gene virulence |
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