| 白肋烟转基因育种研究 |
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| 引用本文： | 林国平,戚华雄,查中萍,王毅,肖宗友,周永碧,黄文昌.白肋烟转基因育种研究[J].湖北农业科学,2005(6):14-16. |
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| 作者姓名： | 林国平 戚华雄 查中萍 王毅 肖宗友 周永碧 黄文昌 |
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| 作者单位： | 1. 湖北省烟草科学研究所/中国烟草白肋烟试验站,武汉,430030
2. 湖北省农业科学院粮食作物研究所,武汉,430064 |
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| 基金项目： | 国家烟草专卖局“优质抗病白肋烟新品种选育”项目（110200202004） |
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| 摘 要： | 利用农杆菌介导法将具有广谱抗病的葡萄糖氧化酶(Glucose Oxidase,GO)基因及抗虫半夏凝集素(Pinella Ternata Agglutinin,PTA)基因转化到烟草中,并研究了影响转化和抗性植株再生的一些因素。结果表明,基因型是影响转化和抗性植株再生的关键因素,试验所用的3个品种KY14、KY8959、TN90中,KY14转化效率最高。用于烟草转化的侵染时间以15min为宜,共培养时间以2d最佳。PCR分子检测表明,外源GO基因及PTA基因已转入到烟草KY14中。淀粉-碘化钾显色反应检测到了转基因植株产生的过氧化氢,证实GO基因已经在烟草中发挥功能。
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| 关 键 词： | 白肋烟 葡萄糖氧化酶基因 半夏凝集素基因 根癌农杆菌 过氧化氢 |
| 文章编号： | 0439-8114(2005)06-0014-03 |
| 收稿时间： | 04 25 2005 12:00AM |
| 修稿时间： | 2005年4月25日 |
Research on the Breeding of Transgenic Burley Tobacco |
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| LIN Guo-ping,QI Hua-xiong,ZHA Zhong-ping,WANG Yi,XIAO Zong-you,ZHOU Yong-bi,HUANG Wen-chang.Research on the Breeding of Transgenic Burley Tobacco[J].Hubei Agricultural Sciences,2005(6):14-16. |
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| Authors: | LIN Guo-ping QI Hua-xiong ZHA Zhong-ping WANG Yi XIAO Zong-you ZHOU Yong-bi HUANG Wen-chang |
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| Institution: | 1.Tobacco Research Institute of Hubei Provinee/Burley Tobacco Experimental Station of CNTC, Wuhan 430030, China; 2. Food Crops Institute of Hubei Academy of Agricultural Sciences, Wuhan 430064, China |
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| Abstract: | This paper studied the factors that influenced the tobacco transformation and the regeneration of plant with resistance by Agrobacterium tumerfaciens.The results showed that the genotypes was the key factor that influenced transformation.KY14 was easier to obtain resistant-regenerated plants than other two varieties tested in this study.The most optimal influence time and co-culture time was 15 min and 2d respectively.The integration of GO and PTA gene into tobacco was confirmed by PCR analysis.The H2O2 generated by the transgenic plants was detected by color reaction with starch-KI and this proved that the glucose oxidase generated by the expression of Go gene had its function in tobacco. |
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| Keywords: | burley tobacco glucose oxidase Pinella ternata agglutinin agrobacterium tumerfaciens hydrogen peroxide |
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