| 花生根瘤菌遗传多样性的RFLP分析 |
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| 引用本文： | 康凯,徐艳,李友国,周俊初.花生根瘤菌遗传多样性的RFLP分析[J].湖北农业科学,2007,46(5):677-680. |
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| 作者姓名： | 康凯 徐艳 李友国 周俊初 |
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| 作者单位： | 华中农业大学农业微生物国家重点实验室,武汉,430070 |
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| 摘 要： | 利用16S rRNA PCR-RFLP和16S-23S rRNA IGS PCR-RFLP技术对分离自我国不同地区的55株花生慢生根瘤菌和6株参比菌株进行了遗传多样性研究.16S rRNA PCR-RFLP分析结果表明,在63%的相似性水平上,91%的供试花生幔生根瘤菌与B.japonicum和B.elkanii聚在一起;16S-23S rRNA IGS PCR-RFLP分析结果表明,供试花生慢生根瘤菌具有较丰富的遗传多样性,在65.8%的相似性水平上可分为3大类群.
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| 关 键 词： | 花生慢生根瘤菌 16S rRNA PCR-BFIP 16S-23S rRNA IGS PCR-RFLP 遗传多样性 |
| 文章编号： | 0439-8114（2007）05-0677-03 |
| 修稿时间： | 2007-05-22 |
Study of Genetic Diversity in Peanut Bradyrhizobia by Rflp Technique |
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| KANG Kai,XU Yan,LI You-guo,ZHOU Jun-Chu.Study of Genetic Diversity in Peanut Bradyrhizobia by Rflp Technique[J].Hubei Agricultural Sciences,2007,46(5):677-680. |
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| Authors: | KANG Kai XU Yan LI You-guo ZHOU Jun-Chu |
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| Institution: | Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology, Wuhan 430070, China |
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| Abstract: | Fifty five rhizobium strains isolated from peanut(Arachis hypogaea) and six reference strains were analyzed by the method of 16S rRNA PCR-RFLP and 16S-23S IGS PCR-RFLP.The result of the 16S rRNA PCR-RFLP showed that 91% of strains tested were ascribed to the genus of Brady rhizobiun phylogenetically at the 63% similarity.Results of 16S-23S IGS PCR-RFLP refelected that the genetic diversity of the strains tested were very high and all the strains tested can be divided into three group at the 65.8% similarity. |
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| Keywords: | Bradyrhizobium sp Arachis 16S rRNA PCR-RFLP 16S-23S rRNA IGS PCR-RFLP genetic diversity |
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