| 质粒DNA提取与酶切方法的比较研究 |
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| 引用本文： | 杜长城,弓维钧,黄俊轩,吕云,杨静慧,赵亚平.质粒DNA提取与酶切方法的比较研究[J].天津农业科学,2006,12(4):1-2. |
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| 作者姓名： | 杜长城 弓维钧 黄俊轩 吕云 杨静慧 赵亚平 |
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| 作者单位： | 1. 天津市林业局林业处,天津,300203
2. 天津农学院园艺系,天津,300384 |
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| 基金项目： | 天津市自然科学基金;天津市科技计划;天津市农业科技成果转化基金 |
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| 摘 要： | 比较了质粒DNA小量和大量制备方法,分析了质粒纯度、酶的种类、单酶切和双酶切方法对酶切效果的影响。结果显示,质粒DNA小量制备法效果好,提取的质粒量大,质量高;GalUR/TEM-T质粒双酶切时需先经过纯化才能酶切完全;BamHI单酶切FRO2/PCB302质粒效果比较好,而XbaI单酶切FRO2/PCB302质粒效率比较低,需要纯化和降低酶切反应中质粒的浓度。
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| 关 键 词： | 质粒 DNA 质粒纯化 酶切 |
| 文章编号： | 1006-6500(2006)04-0001-02 |
| 修稿时间： | 2006年5月11日 |
Methods Research of Distilling and Enzyme-cut Plasmid DNA |
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| DU Chang-cheng,GONG Wei-jun,HUANG Jun-xuan,LV Yun,YANG Jing-hui,ZHAO Ya-ping.Methods Research of Distilling and Enzyme-cut Plasmid DNA[J].Tianjin Agricultural Sciences,2006,12(4):1-2. |
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| Authors: | DU Chang-cheng GONG Wei-jun HUANG Jun-xuan LV Yun YANG Jing-hui ZHAO Ya-ping |
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| Abstract: | To distill plasmid DNA effectively,two methods were compared(small and large quantity).The enzyme-cut efficiency was researched by plasmid purification,single or double enzyme-cut method,different kind of enzyme.The result showed that small quantity method was better with high quality and quantity.After purified the GalUR/TEM-T vectors,their enzyme-cut reaction was completely.The enzyme-cut efficiency of FRO2/PCB302 by BamHI was better than XbaI.If XbaI enzyme cut FRO2/PCB302,the plasmid should be purified at first step and lower the content. |
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| Keywords: | DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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