| 非洲紫罗兰组培技术研究 |
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| 引用本文： | 何家涛.非洲紫罗兰组培技术研究[J].天津农业科学,2006,12(1):20-22. |
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| 作者姓名： | 何家涛 |
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| 作者单位： | 襄樊职业技术学院生物工程系,湖北,襄樊,441021 |
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| 基金项目： | 湖北省襄樊市科技局立项项目 |
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| 摘 要： | 以非洲紫罗兰幼嫩叶片为外植体,用MS作基本培养基,添加不同浓度BA、NAA、GA等探讨对其愈伤组织诱导、不定芽分化、继代增殖及不定根形成的影响,并筛选了适宜的激素水平范围。试验表明:采用MS+0.1～2.0m gL/6-BA+0.01～0.5mgL/NAA培养基,愈伤诱导率在20%以上;采用M S+0.1～1.0mgL/6-BA+0.01～0.5mgL/NAA+0.1～1.0mgL/GA培养基对芽的生长及增殖效果好,并提出GA具有提高不定芽长势和成苗率的作用;采用1/2M S+0.1～0.5mgL/NAA或0.1mgL/IBA或0.1～0.5mgL/IAA,20d内生根率可达97%以上。在腐叶土∶珍珠岩=5∶3的基质中驯苗,成活率可达90%以上。
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| 关 键 词： | 非洲紫罗兰 幼嫩叶片 组织培养 |
| 文章编号： | 1006-6500（2006）01-0020-03 |
| 收稿时间： | 2005-07-25 |
| 修稿时间： | 2006-02-22 |
Research on Tissue Culture Technique of Saintpaulia lonatha Wendl |
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| HE Jia-tao.Research on Tissue Culture Technique of Saintpaulia lonatha Wendl[J].Tianjin Agricultural Sciences,2006,12(1):20-22. |
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| Authors: | HE Jia-tao |
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| Institution: | Department of Biological Engineering, XiangFan Vocational and Technical College, XiangFan, Hubei 441021, China |
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| Abstract: | With saintpaulia ionatha wendl leaflet as explants and MS as basic culture medium,multiplication cultivation and culturing seedings of saintpaulia ionatha wendl were tested in 6-BA?NAA?GA at different density.While MS+0.1～2.0 mg/L BA+0.01～0.5 mg/L NAA were applied as culture medium,the rate is greater than 20%.In MS+0.1～1.0 mg/L 6-BA+0.01～0.5 mg/L NAA+0.1～1.0 mg/L GA medium,it had good multiplication effect and growth of sprouts.GA promoted indefinite bud growth and seeding rate.With medium of 1/2MS+0.1～0.5 mg/L NAA or 0.1 mg/L IBA or 0.1～0.5 mg/L IAA makes the rooting rate of 97% in 20 days;the survival rate would exceed 90% when the sprouts were transplanted into the 5∶3 humus soil and perlite substrate. |
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| Keywords: | saintpaulia ionatha wendl lender leaf tissue culture |
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