杂种黄秋葵茎尖及试管苗培养的研究

采用茎尖和试管苗培养的方法,以黄秋葵生长点为材料,进行了生长点的生长、分化芽的生根和试管苗的生根继代增殖培养,以及试管苗的移栽与定植的研究。结果表明:1/2MS+ZT0.2mg·L-1+NAA0.3mg·L-1是生长点伸长生长的理想培养基;MS+6-BA0.7mg·L-1+AgNO30.8mg·L-1+NAA0.1mg·L-1是生长芽分化培养的理想培养基;1/3MS+ABT0.6mg·L-1+蔗糖10g·L-1+IAA0.4mg·L-1是分化芽生根培养和生根继代增殖培养的理想培养基。试管苗移栽成活率为94.7%;定植成活率为97.4%;定植的试管苗保持了杂种黄秋葵的杂种优势。

Study on Propagation for Tender Buds and Tube Seedlings of Abelmoschus esculentus

Using the culture methods of shoot tip and tube seedlings, the Abelmoschus esculentus growing point for the material, the study was mainly on growth of growing points, differentiation of differentiation bud, rooting and subculturing of tube seedlings, and tube seedlings transplanting colonization. The results demonstrated that: 1/2MS + ZT 0.2 mg·L ~(-1) + NAA 0.3 mg·L~( -1 )was the optimum medium for the elongation growth of growing points; MS+6-BA 0.7 mg·L~( -1) + AgNO 3 0. 8 mg·L -1 + NAA0.1 mg·L~( -1) was the suitable medium for differentiation cultivation of growth bud; 1/3MS + ABT 0.6 mg·L~( -1) + sucrose 10 g·L~( -1 )+ IAA 0.4 mg·L~( -1) was the optimum medium for differentiation, rooting and subculture of differentiation bud. The transplanting survival rate of tube seedlings was 94.7% and stable planting survival rate was 97.4%. Colonization of plantlets maintained the heterosis of Abelmoschus esculentus.