| 茜素紫与牛血清白蛋白相互作用特征研究 |
| |
| 引用本文： | 汪永红,吴刚珂,颜承农.茜素紫与牛血清白蛋白相互作用特征研究[J].长江大学学报,2009(4). |
| |
| 作者姓名： | 汪永红 吴刚珂 颜承农 |
| |
| 作者单位： | 荆州市中心医院检验科;长江大学化学与环境工程学院;长江大学工程技术学院; |
| |
| 基金项目： | 湖北省自然科学基金项目(2005ABA067) |
| |
| 摘 要： | 在不同温度下,用荧光光谱、同步荧光光谱、三维荧光光谱和紫外可见吸收光谱法研究了茜素紫(Alizarin-Violet,ALV)与牛血清白蛋白(bovineserum albumin,BSA)相互作用特征。分别用Stern-Volmer方程、Lineweaver-Burk双倒数方程和热力学方程等处理试验数据,得到相互作用常数的平均值为1.078×105L/mol,相互作用的ΔHθ、ΔGθ和ΔSθ的平均值分别为-8.030kJ/mol、-29.19kJ/mol和69.80J/K,作用位置为2.38nm,作用位点数为1.173。证实了在试验浓度和温度范围内,ALV与BSA可相互作用形成具有一定结构的复合物,其荧光猝灭作用更符合静态猝灭作用特征,为研究ALV的生态环境效应、生物学效应和对蛋白质构象的影响以及ALV的染色机理等提供了重要信息。
|
| 关 键 词： | 茜素紫 牛血清白蛋白 荧光猝灭光谱 三维荧光光谱 吸收光谱 热力学参数 |
Study on the Action Features between Alvizarin-violet and Bovine Serum Albumin by Fluorescence Spectrophtometry |
| |
| WANG Yong-hong WU Gang-ke YAN Cheng-nong.Study on the Action Features between Alvizarin-violet and Bovine Serum Albumin by Fluorescence Spectrophtometry[J].Journal of Yangtze University,2009(4). |
| |
| Authors: | WANG Yong-hong WU Gang-ke YAN Cheng-nong |
| |
| Institution: | WANG Yong-hong WU Gang-ke (Jingzhou Centeral Hospital,Jingzhou 434020)YAN Cheng-nong (Yangtze University,Jingzhou 434023) |
| |
| Abstract: | Under the different temperatures,the binding of alizarin-violet to bovine serum albumin (BSA) was studied by the fluorescence spectroscopy,three-dimension,synchronous fluorescence spectrum and ultra-violet spectrum.After analyzing the fluorescence quenching data according to Sterm-Volmer equation,Lineweaver-Burk equation and thermodynamic equation,BSA was reacted with Alizarin-Violet and a new compound was found,the quenching belonged to static fluorescence quenching,action force was electro- static interac... |
| |
| Keywords: | Alizarin-Violet Bovine serum albumin Fluorescence spectrum Three dimensional fluorescence spectrum Ultra-violet spectrum Thermodynamic parameters |
| 本文献已被 CNKI 等数据库收录！ |
