胃肠道恶性肿瘤中Runx3基因甲基化研究

目的：通过检测胃肠道恶性肿瘤细胞系中Runx3基因启动子区域甲基化状态及Runx3基因表达情况，探讨Runx3基因启动子区域甲基化及表达在胃肠道恶性肿瘤中发生和发展过程中的意义。方法：采用DNA甲基化特异性PCR（MSP）技术分别对9种胃癌细胞系和11种结直肠癌细胞系中Runx3基因启动子区域甲基化进行检测，同时采用逆转录一聚合酶链反应（RT—PCR）检测Runx3mRNA的表达情况。结果：在7种胃癌细胞系和6种结直肠癌细胞系中，Runx3基因启动子区域过度甲基化；RT—PCR结果显示，在20种胃肠道恶性肿瘤细胞系中有15种细胞系Runx3基因未表达。结论：Runx3基因启动子区域甲基化是导致Runx3基因失活的主要原因之一，与胃肠道恶性肿瘤的发生发展密切相关，可作为胃肠道恶性肿瘤早期诊断的分子标记物及分子治疗的靶点。

Analysis on Promoter Methylation of Runx3 Gene in Gastric and Colorectal Cancer

Objective:To study the clinic significance of Runx3 gene hypermethylation and expression in Gastric and colorectal cancer,we examined the promoter methylation status and expression of Runx3 gene in cell lines.Methods:The methylation status of Runx3 was examined by methylation-specific polymerase chain reaction(MSP).Meanwhile,RT-PCR was used to detect expression of Runx3 gene in the Gastric and colorectal cancer cell lines.Results:Hypermethylation was observed in the promoter region of seven gastric cell lines and six colorectal cell lines.The RT-PCR results indicate Runx3 mRNA was not expressed in fifteen kinds of cell lines.Conclusion:High frequent hypermethylation was one of reasons which induced Runx3 gene inactivation in human gastric and colorectal cancer,hypermethylation of Runx3 promoter may be correlated to on cogenesis of gastric and colorectal cancer.Runx3 gene can be utilized as a molecular diagnostic marker and therapy target of gastric and colorectal cancer.