肉苁蓉对感染性休克肝线粒体功能影响的实验研究

目的:研究肉苁蓉对感染性休克肝线粒体功能的影响,探讨肉苁蓉在细胞水平治疗感染性休克的机制.方法:40只SD大鼠随机分为5组:假手术组,12 h对照组,12 h给药组,16 h对照组和16 h给药组.采用盲肠结扎穿孔术(CLP)复制感染性休克模型,比较各组存活率和平均动脉压(MAP),肝线粒体ATP酶活性,呼吸控制率(RCR),ADP/O比值的变化.结果:大鼠在复制感染性休克模型后,肝线粒体ATP酶活性,呼吸控制率和ADP/O比值显著降低,24 h存活率明显下降.给药组肝线粒体ATP酶活性,呼吸控制率(RCR)和ADP/O比值较对照组明显改善(P＜0.05),与大鼠死亡率的降低呈明显正相关.结论:肉苁蓉能增强线粒体摄氧功能和氧化磷酸化功能,恢复感染性休克线粒体的正常的能量代谢功能.

Studies on effect of Cistanche deserticola Y.C.Ma on the function of liver mitochondria following septic shock

Objective: study on effect of Cistanche deserticola Y.C.Ma on the function of liver mitochondria following septic shock.Illustrate on michanism of Cistanche deserticola Y.C.Ma treatment for septic shock.Methods: 40 rats were randomly divided into 5 groups:artificial groups, 12h control groups, 12h Cistanche deserticola Y.C.Ma groups, 16h control groups, 16h Cistanche deserticola Y.C.Ma groups.The model of septic shock was made by cecal ligation and puncture.Cistanche deserticola Y.C.Ma and saline were via gastric garage to the rats respectively for fifteen days.At 4h,6h,8h,feed them once after operation.Liver mitochondria RCR,P/O,ATPase activity were assayed.Results: Liver mitochondria RCR ,P/O and ATPase activity were obviously decreased after CLP.However,in the Cistanche deserticola Y.C.Ma groups,All of them were significantly better than control group(P<0.01),Furthermore keep positive correlation with improvement of death rate.Conclusion: Cistanche deserticola Y.C.Ma can strengthen the function of liver mitochondrial ingestion-oxygen and energy metabolization,and effectively attenuate the dysfunction of hepatocytic mitochondria from the damage induced by septic shock.