3种鱼类致病菌粗脂多糖(LPS)对斑点叉尾鮰的免疫原性

用温酚法分别从柱状嗜纤维菌(Cytophaga columnaris)、嗜水气单胞菌(Aeromonas hydrophila)和叉尾鮰爱德华菌(Edwardsiella ictaluri)3种鱼类致病菌中提取粗脂多糖(LPS)作为免疫原,分别接种斑点叉尾鮰(Ictalurus punctatusRafinsque)后,通过测定受免鱼的交叉凝集抗体效价、头肾和血液中吞噬细胞的吞噬活性以及对A.hydrophila活菌细胞内的杀菌活性,探讨了3种致病菌的粗LPS对斑点叉尾鮰的免疫原性。结果表明,从3种致病菌中提取的粗LPS对斑点叉尾鮰均具有较强的免疫原性,受免鱼的血清中存在对3种致病菌的交叉凝集抗体,与对照鱼相比,头肾和血液中吞噬细胞对吞噬原的吞噬活性和对A.hy-drophila活菌杀菌活性明显上升。说明在3种供试菌的粗LPS上不同程度地存在3种致病菌的共同保护性抗原。

Immunogenicity of Crude Lipopolysaccharide of Three Pathogenic Bacteria to Channal Catfish,Ictalurus punctatus

Channal catfish(Ictalurus punctatus Rafinsque)were immunized with crude lipopolysaccharide(LPS) extracted from Cytophaga columnaris,Aeromonas hydrophila and Edwardsiella(ictaluri).The immunogenicity of crude lipopolysaccharide to channal catfish were compared by examining the agglutinating antibody titers,cross-agglutinating antibody titers of serum,the phagocytic activity and bactericidal activity of phagocytes in the blood and head kidney.The results showed that the agglutinating antibody titers and cross-agglutinating antibody titers of immunized channel catfish were higher than the control group,and the phagocytic activity and significantly boosted and had a high level of immune response.The crude LPS of three pathogenic bacteria were thought to have a common protective antigen which cause a cross-immune response to channel catfish.