| DNA-AFLP分析用辣椒基因组DNA提取方法的优化 |
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| 引用本文： | 向珣,宋小丽,施倩倩,柴伟国,曹家树.DNA-AFLP分析用辣椒基因组DNA提取方法的优化[J].长江大学学报,2009(2). |
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| 作者姓名： | 向珣 宋小丽 施倩倩 柴伟国 曹家树 |
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| 作者单位： | 浙江大学农业与生物技术学院;农业部"园艺植物生长发育与品质调控"重点开放实验室;浙江省杭州市农业科学研究院; |
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| 基金项目： | 浙江省自然科学基金项目(Y3080128);;国家自然科学基金项目(30771377) |
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| 摘 要： | 采用SDS法、CTAB-1法和CTAB-2法提取辣椒(Capsicu mannuum)叶片DNA,通过紫外吸收和琼脂糖凝胶电泳检测,证明CTAB-2法所提取DNA的质量和纯度较高;以此DNA为模板进行DNA-AFLP分析,特征条带清晰、基本无脱尾,适用于DNA-AFLP等分子生物学研究。
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| 关 键 词： | CTAB 辣椒（Capsicu mannuum） DNA DNA-AFLP |
Optimization of Genomic DNA Extraction Methods to DNA-AFLP in Capsicum annuum |
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| XIANG Xun,SONG Xiao-liSHI Qian-qian College of Agriculture , Biotechnology,Zhejiang University,The StateAgriculture Ministry Laboratory of Horticultural Plant Growth , QualityRegulation,Hangzhou,Zhejiang ,ChinaCAI Wei-guo CAO Jia-shu College of Agriculture , Biotechnology of Zhejiang University,The State Agriculture MinistryLaboratory of Horticultural Plant Growth , Quality Regulation,China.Optimization of Genomic DNA Extraction Methods to DNA-AFLP in Capsicum annuum[J].Journal of Yangtze University,2009(2). |
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| Authors: | XIANG Xun SONG Xiao-liSHI Qian-qian College of Agriculture Biotechnology Zhejiang University The StateAgriculture Ministry Laboratory of Horticultural Plant Growth QualityRegulation Hangzhou Zhejiang ChinaCAI Wei-guo CAO Jia-shu College of Agriculture Biotechnology of Zhejiang University The State Agriculture MinistryLaboratory of Horticultural Plant Growth Quality Regulation China |
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| Institution: | Hangzhou Academy of Agricultural Sciences;Zhejiang;310024;China |
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| Abstract: | DNA extraction is a basic technique in molecular biology. The purity and integrality of DNA structure are necessary for different experiments of gene engineering. SDS and CTAB methods were used to extract DNA from hot pepper leaves.The extraction method of CTAB were optimized.The results of UV-sepectrophotometry and gel electrophoresis showed that DNA extracted by CTAB-2 method was suitable for hot pepper. DNA-AFLP with DNA extracted by CTAB-2 as templates showed that its amplification was good,clear and un... |
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| Keywords: | CTAB Capsicum annuum DNA extraction DNA-AFLP |
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