核桃缘吉丁成虫的取食选择行为及机制

  目的  通过比较核桃缘吉丁成虫对核桃、苹果和山楂这3种植物在取食选择和取食量上的差异，并分析植物挥发物的组成与含量，该研究为进一步开发核桃缘吉丁植物源引诱剂提供了依据。  方法  测定核桃缘吉丁成虫对核桃、苹果和山楂叶片的取食选择和取食量，采用动态顶空法收集这3种植物的枝叶挥发物组分及相对含量的测定，通过卡方检验分析比较核桃缘吉丁对植物挥发物的行为反应。  结果  核桃缘吉丁成虫对不同植物的取食选择和取食量存在显著差异，成虫对核桃的取食选择性最高且取食核桃叶片，对苹果和山楂的选择性较低，且存在不取食现象。从核桃、苹果和山楂的枝叶挥发物中，共收集到45种植物挥发性化合物，包括烷烃类5种、酯类8种、芳香烃类3种、萜类18种、醛类2种、醇类4种和酮类5种。在行为反应测定中，莰烯对核桃缘吉丁雌雄成虫均具有明显的趋向反应，而乙酸冰片酯、癸醛和桉叶油醇这3种单体化合物仅对雄虫具有一定的引诱效果。  结论  核桃可作为核桃缘吉丁成虫补充营养的寄主植物，该成虫不取食苹果和山楂。莰烯、乙酸冰片酯、癸醛和桉叶油醇对核桃缘吉丁成虫具有一定的吸引效果，需要重点关注，这些枝叶挥发物成分在核桃缘吉丁成虫寄主植物识别与选择的阶段中可能发挥着重要作用。 

Feeding choice behavior and mechanism of adult Meliboeus ohbayashii primoriensis

  Objective  By comparing differences in the selection and consumption of Meliboeus ohbayashii primoriensis to 3 kinds of plants (Juglans regia, Malus pumila and Crataegus pinnatifida), and by analyzing the composition and content of plant volatiles, this research provides a solid basis for a further development of the plant source attractant for M. ohbayashii primoriensis.  Method  The leaves of three plants (J. regia, M. pumila and C. pinnatifida) were used to determine host plant choice and consumption by this insect pest. Volatile components in the branches and leaves of these three plants were collected using the dynamic headspace method and were analyzed by gas chromatography-mass spectrometry. Furthermore, Chi-square test was used to analyze and compare the differences in behavioral responses of M. ohbayashii primoriensis adults in the volatiles emitted by these plants.  Result  Significant differences were found in the selection and consumption of the three plants by M. ohbayashii primoriensis. Adults displayed high feeding selectivity for J. regia and consumed J. regia leaves. Whereas they showed low feeding selectivity for M. pumila and C. pinnatifida, without consuming their leaves. A total of 45 plant volatile compounds were identified from the branches and leaves of these three plants, including five alkanes, eight esters, three aromatic hydrocarbons, 18 terpenes, two aldehydes, four alcohols and five ketones. In the experiment of behavioral responses, both male and female adults of M. ohbayashii primoriensis were attracted by camphene in obvious tendency responses, while only males were attracted by bornyl acetate, decanal and eucalyptol in a certain attraction.  Conclusion  J. regia can be used as a host plant to supplement the nutrition of adult M. ohbayashii primoriensis, whereas the leaves of M. pumila and C. pinnatifida are not suitable for consumption. Camphene, bornyl acetate, decanal and eucalyptol had a certain attraction effect on the adults M. ohbayashii primoriensis. These volatile components may play an important role during the identification and selection of host plants by adult M. ohbayashii primoriensis. These volatile components of branches and leaves may play an important role during the identification and selection of host plants by adult M. ohbayashii primoriensis.