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神农架4个珙桐居群遗传多样性的RAPD分析
引用本文:李雪萍,何正权,陈发菊,梁宏伟,李凤兰.神农架4个珙桐居群遗传多样性的RAPD分析[J].北京林业大学学报,2006,28(3):66-70.
作者姓名:李雪萍  何正权  陈发菊  梁宏伟  李凤兰
作者单位:1.北京林业大学生物科学与技术学院
基金项目:湖北省教育厅科研项目 , 三峡大学校科研和校改项目
摘    要:用随机扩增多态DNA(RAPD)技术对神农架地区4个居群28个珙桐(Davidia involucrate Baill.)个体进行了遗传多样性分析.16个10 bp寡核苷酸引物共检测到87个位点,多态位点49个,多态百分率56.3%,而居群平均多态位点百分率为30.2%.用POPGENE对数据进行了分析,结果显示:居群的平均Nei’s基因多样性为0.111 8,Shannon’s遗传多样性信息指数为0.165 1,总的居群基因多样性为0.169 2,基因分化系数为0.339 2.这表明神农架地区珙桐的遗传多样性较低,其遗传变异以居群内遗传变异为主. 

关 键 词:珙桐    遗传多样性    RAPD
文章编号:1000-1522(2006)03-0066-05
收稿时间:04 5 2005 12:00AM
修稿时间:2005-04-05

RAPD analysis for the genetic diversity of four populations of Davidia involucrate Baill. in Shennongjia area, Hubei Province
LI Xue-ping,HE Zheng-quan,CHEN Fa-ju,LIANG Hong-wei,LI Feng-lan.RAPD analysis for the genetic diversity of four populations of Davidia involucrate Baill. in Shennongjia area, Hubei Province[J].Journal of Beijing Forestry University,2006,28(3):66-70.
Authors:LI Xue-ping  HE Zheng-quan  CHEN Fa-ju  LIANG Hong-wei  LI Feng-lan
Institution:1, College of Biological Science and Biotechnology, Beijing Forestry University, 100083, P. R. China; 2, Biotechnology Research Center, China Three Gorges University, Yichang, Hubei Province, 443002, P. R. China
Abstract:Twenty-eight individuals of four Davidia involucrata Baill.populations in Shennongjia area,Hubei Province were analyzed by using random amplified polymorphic DNA(RAPD) markers to determine the genetic variation among and within the populations.Eighty-seven loci were detected using sixteen 10 bp random primers,of which 49 were exhibited polymorphism.The average percentage of polymorphic loci(PPL) was 30.2%,while that of polymorphic loci(PPL) was 56.3%.Data were analyzed by the software POPGENE and results showed that the average Nei's genetic diversity was 0.111 8,Shannon's genetic diversity information index was 0.165 1,genetic diversity of all populations was 0.169 2 and Gst was 0.339 2.The results indicate that D.involucrata in this area has relatively low genetic diversity and most of differentiation is within populations.
Keywords:Davidia involucrata Baill    genetic diversity  RAPD
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