黄绿木霉菌代谢产物对杨树烂皮病菌抑菌能力的研究

该文利用室内实验的方法，研究了黄绿木霉菌代谢产物对杨树烂皮病菌的抑菌能力.黄绿木霉菌代谢产物在经121℃处理25 min后，50%浓度下抑菌作用可高达100%.在pH为3～9范围内该代谢产物的抑菌能力变化不大，说明pH变化对其活性没有影响.在对黄绿木霉菌发酵过程中，利用马铃薯葡萄糖(PD)培养基即可达到有效的抑制杨树烂皮病金黄壳囊孢菌菌丝生长的目的，而且利用马铃薯葡萄糖(PD)培养基发酵3～4 d的黄绿木霉菌代谢产物抑菌效果最好，抑菌中浓度最低，发酵时间过长与过短，均不利于对病原菌的抑制.经黄绿木霉菌代谢产物处理的菌丝电导率与呼吸强度均发生变化，在光学显微镜与透射电镜下观察到病原菌菌丝体发生变化，细胞壁有所改变. 

Study on inhibition pathogen of Cytospora chrysosperma by Trichoderma aureoviride metabolite

Through the indoor trial methods, the ability of inhibiting Cytospora chrysosperma by Trichoderma aureoviride was studied. After treated at 121℃ for 25 min and diluted one fold, the metabolite performed 100% inhibiting ability to mycelium growth. This inhibition ability did not change significantly within the range from pH 3 to pH 9. It indicated that inhibition ability did not relate to the changing of pH value. During the process of fermentation of T. aureoviride, single PD medium could efficiently inhibit mycelium growth of C. chrysosperma. The metabolite of T. aureoviride showed the best inhibition ability and lowest LC-50 after culturing for 3-4 days with PD medium. Shorter or longer periods of fermentation all had no benefits on inhibiting pathogen. The conductivity and respire intensity of mycelium treated by T. aureoviride metabolite were also changed. Changes of mycelium and cell wall of the pathogen were observed through optics microscope and TEM.