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GC-MS内标法定量分析植物组织中的JA、IAA和ABA
引用本文:李金克,陈华君,陈少良.GC-MS内标法定量分析植物组织中的JA、IAA和ABA[J].北京林业大学学报,2010,32(5):143-148.
作者姓名:李金克  陈华君  陈少良
作者单位:1北京林业大学实验室与设备管理处 2林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室,国家林业局树木花卉育种与生物工程重点开放实验室,北京林业大学生物科学与技术学院3湖北民族学院生物资源保护与利用湖北省重点实验室
基金项目:国家自然科学基金,"863"国家高技术研究发展计划项目,北京市优秀博士学位论文指导教师科技项目,教育部重点项目,全国高校优秀博士学位论文作者专项基金,高等学校优秀青年教师教学科研奖励计划,霍英东教育基金会高等院校青年教师基金,湖北省自然科学基金 
摘    要:提出了利用气相色谱-质谱联用仪同时定量测定植物组织中茉莉酸(JA)、吲哚-3-乙酸(IAA)和脱落酸(ABA)的方法。实验以欧洲灰杨叶片为材料,在用80%冷甲醇提取激素时,一并加入ABA、IAA和JA的内标(D3-ABA300ng,13C6-IAA60ng和DHJA80ng),激素提取液浓缩至水相,调pH2.5~3.0后经乙酸乙酯萃取、Sep-PakC18小柱纯化,甲酯化后,GC-MS定量测定ABA、JA。IAA经甲酯化后再经TMS化(三甲基硅烷化),用GC-MS定量测定。经检测:盆栽欧洲灰杨叶组织中脱落酸含量为120.170~128.771ng/g(鲜质量),茉莉酸含量为1.697~6.974ng/g(鲜质量),吲哚乙酸含量为4.990~14.776ng/g(鲜质量);脱落酸含量最高,其次是吲哚乙酸,茉莉酸含量最低。

关 键 词:气相色谱--质谱联用仪    内标法    茉莉酸    吲哚乙酸    脱落酸    欧洲灰杨
收稿时间:1900-01-01

Quantitative analysis of jasmonic acids, indole-3-acetic acid and abscisic acid in plant tissues by GC-MS
LI Jin-ke,CHEN Hua-jun,CHEN Shao-liang.Quantitative analysis of jasmonic acids, indole-3-acetic acid and abscisic acid in plant tissues by GC-MS[J].Journal of Beijing Forestry University,2010,32(5):143-148.
Authors:LI Jin-ke  CHEN Hua-jun  CHEN Shao-liang
Institution:LI Jin-ke1,CHEN Hua-jun1,CHEN Shao-liang2,3 1 Laboratory and Equipment Administration Department,Beijing Forestry University,100083,P.R.China,2 National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,College of Biological Sciences and Biotechnology,3 Key Laboratory of Biol...
Abstract:GC-MS was used to determine the concentrations of jasmonic acid (JA), indole-3-acetic acid (IAA) and abscisic acid (ABA) in Populus canescens leaves in this paper. Abundant cold methanol (80%, v/v) was used to extract JA, IAA and ABA, and three internal standards, D3-ABA 300 ng,13C6-IAA 60 ng and DHJA 80 ng were simultaneously introduced into the leaf samples. Crude extract was condensed by vacuum evaporating and hormones were re-extracted by ethyl acetate at pH 2.5--3.0 (×3). Then condensed JA, IAA and ABA extracts were purified by Sep-Pak C18 columns. Thereafter, partially purified hormones were methylated using trimethylsilyldizomethane and then analyzed by GC-MS (JA and ABA). IAA was analyzed by GC-MS after an additional TMS reaction. Results showed that concentrations of ABA, JA, and IAA in leaf tissues of P. canescens were 121.064--128.771 ng/g, 2.190--6.974 ng/g and 4.990--15.012 ng/g Fw, respectively. In concentration in P. canescens leaves, ABA > IAA> JA.
Keywords:GC-MS  internal standard method  jasmonic acid  indole-3-acetic acid  abscisic acid  Populus canescens
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