不同2n雌配子来源的青杨杂种三倍体与其亲本蛋白质组差异研究

目的研究两种2n雌配子来源的青杨杂种三倍体与亲本的蛋白质组表达差异，从蛋白水平探讨异源三倍体杨树在生长、抗性等方面具有优势的分子基础，为杨树多倍体选育和遗传改良提供科学依据。方法采用同位素标记相对和绝对定量(iTRAQ)技术对青杨三倍体与其亲本进行定量蛋白质组学研究，所提取的杨树蛋白质样品经FASP酶解、iTRAQ试剂标记、高pH-RPLC分离、RPLC-MS分离分析，获取的串联质谱数据通过软件Proteome Discoverer 1.3搜库进行蛋白质鉴定，通过蛋白质相对定量的比较寻找差异表达蛋白，再对差异蛋白质进行GO、KEGG代谢通路分析。结果本研究共鉴定出1 472个蛋白质，差异蛋白202个。FDR和SDR青杨杂种三倍体与母本‘哲引3号杨’、父本‘北京杨’的差异蛋白比率在2.0%~10.1%之间。两种不同2n配子来源三倍体中FDR与亲本差异蛋白比率最高，且两种三倍体与父本的差异蛋白比率均比母本高。通路注释分析显示，差异蛋白显著富集于代谢相关、核糖体组装、光合作用和胁迫响应等通路。结论杂交和加倍后促进了蛋白质的合成以及光合作用的增强，并提高了多倍体的抗逆性和适应性，这些变化促进了杨树异源多倍体营养生长优势的形成。 

Comparative proteomics of two Populus spp. ( Section Tacamahaca) allotriploid derived by different types of 2 n female gamete and their parents

ObjectiveAlthough Populus allotriploid has a prominent vegetative growth advantages, the underlying molecular mechanisms have not yet been revealed and elucidated. This work was designed to investigate proteins differentially expressed in the two Populus allotriploid derived by different types of 2n female gamete and their parents. The results will provide the scientific foundation for Populus polyploidy selection and genetic improvement.MethodThe iTRAQ proteomics approach was used in this study. The extracted proteins were digested using FASP method and identified by iTRAQ coupled with LC-MS/MS technology. Raw data were analyzed by Proteome Discoverer 1.3 search engine. Then the pathway analysis was conducted using GO and KEGG.ResultA total of 1 472 proteins were identified and 202 proteins were detected as differentially expressed proteins. The ratio of differentially expressed proteins between FDR and SDR Populus allotriploid and the two parents varied from 2.0% to 10.1%. Compared with the female parent or male parent, the ratio of differentially expressed proteins in FDR Populus allotriploid was higher than in SDR Populus allotriploid. In particular, there was an expression level dominance bias toward the triploid progenitors. Further analysis showed that the differentially expressed proteins were significantly enriched in the pathways such as metabolic related, ribosomes, photosynthesis and response to stress.ConclusionThe results indicated that plyploidization and hybridization could enhance photosynthesis, proteins synthetic and increased the resistance and adaptability of Populus polyploidy. All of these contribute to vegetative growth advantages in allotriploid plants.