| 文冠果变异株和野生型植株基因组差示杂交研究初报 |
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| 引用本文： | 彭伟秀,沈昕,李凤兰,白根本.文冠果变异株和野生型植株基因组差示杂交研究初报[J].北京林业大学学报,2000,22(1):29-32. |
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| 作者姓名： | 彭伟秀 沈昕 李凤兰 白根本 |
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| 作者单位： | 1. 河北农业大学林学院
2. 北京林业大学生物学院,100083,北京 |
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| 基金项目： | 国家自然科学基金;39670619; |
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| 摘 要： | 文冠果 (XanthoccrassorbifoliaBunge)同源异型变异株是自然产生的花器官发生变异的个体 .应用扣除杂交技术对该变异株和野生型植株进行了基因组差示杂交 ,获得样本特异DNA片段 ,此DNA片段可以作为进一步筛选突变基因的探针 ,为克隆文冠果花发育调控基因奠定基础
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| 关 键 词： | 文冠果 基因组差示杂交 特异DNA片段 |
Cloning the differences between the wild and mutant genomes of Xanthoceras sorbifolia Bunge |
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| Peng Weixiu,Shen Xin,Li Fenglan,Bai Genben.Cloning the differences between the wild and mutant genomes of Xanthoceras sorbifolia Bunge[J].Journal of Beijing Forestry University,2000,22(1):29-32. |
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| Authors: | Peng Weixiu Shen Xin Li Fenglan Bai Genben |
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| Institution: | Peng Weixiu
,Shen Xin
,Li Fenglan
,Bai Genben
(College of Plant Sciences, Beijing For. Univ., 100083, P. R. China.) |
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| Abstract: | A natural flower homeotic mutant of Xanthoceras sorbifolia Bunge was studied by genome DNA subtraction between the mutant and wild plants. Through the subtraction, a wild plant specific genome DNA fragment has been found and this fragment will be used as a probe in following mutant gene screening study. |
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| Keywords: | Xanthoccras sorbifolia Bunge genome DNA subtraction specific DNA fragment |
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