几种中国兰种子试管培养愈伤组织发生的研究

中国兰种子细小，种皮细胞壁加厚，表面覆盖一层不透水不透气的膜状物质．种胚为发育不完全的球形胚，仅含脂类作为储存营养物质，自然条件下难以萌发．以蕙兰和墨兰的种子进行试管培养，种子萌发后用激素处理可诱导产生愈伤组织，愈伤组织产生于表皮和皮层细胞，由于细胞发育程度不一致，出现胚状体、不定芽、不定根、愈伤组织交织在一起的愈伤组织块，这是种子发育成苗的另一途径．由于愈伤组织可以切割继代培养，也可以进行快速繁殖，但愈伤组织材料以致密愈伤组织为好．

Callus Development of Chinese Orchid Under in vitro Seed Germination

Chinese orchid 's seed is very small. Seed coat is made up of one layer transparent cells whose wall is thickened.It is covered by low penetratable integuments.Globular embryo is undifferentiated.Only lipid is deposited as nutrient.Seed is difficult to germinate in nature. So seeds of Cymbidium faberi and C. sinense were cultured in vitro. Seed was geminated under suitable conditions.Callus were formed from epidemal and cortical cells while protocorm like body (PLB) had been treated with hormones.Embyonic body,adventitious bud and root,callus were often developed simultaneously. Mass propagation is also possible by compact callus culture.