功能基因组学和代谢组学技术在植物次生代谢物合成及调控研究中的应用

植物次生代谢是植物在长期进化过程中与环境相互作用的结果，由初生代谢派生. 萜类、生物碱类、苯丙烷类为植物次生代谢物的主要类型，其代谢途径多以代谢频道形式存在，具有种属、生长发育期等特异性. 该文从植物次生代谢物的分类、代谢途径及代谢调控基因工程等方面展开论述，介绍了次生代谢物的生物合成途径，以及利用基因工程等技术对植物次生代谢途径进行遗传改良等方面的研究进展，为全面认识植物代谢网络、合理定位次生代谢及其关键酶、促进野生植物资源可持续利用等提供理论依据. 

Application of functional genomic and metabolomic techniques to the studies on biosynthesis and regulation of plant secondary metabolites

Plant secondary metabolism is resulted from the interactions between plants and environment during the long-term evolution process,and is derived from the so-called primary metabolism.Terpenoids,alkaloids and phenylpropanoids are the three main types of plant secondary metabolites,whose metabolic pathways mostly exist in a way of metabolic channels,and the pathways possess the characteristics of species,genus and the phase of growth and development.The paper presents the discussions on the classification of plant secondary metabolites,the metabolic pathways and the gene engineering of metabolic regulations,in order to provide theoretical bases for comprehensively understanding the plant metabolism network,their reasonable positioning of secondary metabolism and its key enzymes,and for stimulating the sustainable exploration of wild plant resources.The discussions were emphasized on the biosynthetic pathways of the secondary metabolites and some other aspects including genetic improvement strategies on plant secondary metabolic pathways by gene-engineering technology.The paper also pays much more attention to expound the application perspectives of the functional genomic and metabolomic techniques to the studies on biosynthesis and regulation of plant secondary metabolites.