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超量表达胡杨peu-MIR156j基因增强拟南芥耐盐性
引用本文:段中鑫,覃玉蓉,夏新莉,尹伟伦.超量表达胡杨peu-MIR156j基因增强拟南芥耐盐性[J].北京林业大学学报,2011,33(6):1-7.
作者姓名:段中鑫  覃玉蓉  夏新莉  尹伟伦
作者单位:北京林业大学林木育种国家工程实验室
基金项目:“948”国家林业局引进项目(2007-4-01);国家自然科学基金项目(30730077、30972339、31070597);“十二五”国家科技支撑计划课题(2011BAD38B01);北京市共建项目专项“森林质量升级及高效利用产学研联合研究生培养基地建设”
摘    要:Micro RNAs(miRNAs)是一类内源性的非编码小分子RNA。本文克隆了miR156j的前体(peu-MIR156j),并将其转入拟南芥获得35S:MIR156j转基因植株。对转基因植株的鉴定表明,过量表达miR156j增加了拟南芥莲座叶的发生,导致叶片浓密及开花延迟。这一结果表明:同拟南芥、水稻等物种类似,胡...

关 键 词:胡杨  超表达  耐盐  拟南芥  miR156
收稿时间:1900-01-01

Overexpression of Populus euphratica peu-MIR156j gene enhancing salt tolerance in Arabidopsis thaliana
DUAN Zhong-xin, QIN Yu-rong, XIA Xin-li, YIN Wei-lun.Overexpression of Populus euphratica peu-MIR156j gene enhancing salt tolerance in Arabidopsis thaliana[J].Journal of Beijing Forestry University,2011,33(6):1-7.
Authors:DUAN Zhong-xin  QIN Yu-rong  XIA Xin-li  YIN Wei-lun
Institution:National Engineering Laboratory for Tree Breeding,Beijing Forestry University,100083,P. R. China
Abstract:MicroRNAs (miRNAs) is a class of endogenous non-coding small molecule RNA. To analyze the biological function of Populus euphratica miR156j, miR156j precursor(peu MIR156j)was cloned and transferred into Arabidopsis thaliana, and 35S:MIR156j transgenic plants were obtained in the current work. The results showed that overexpression of miR156j increased the number of rosette leaves, leading to thick leaves and delayed flowering, which was similar to A. thaliana,rice, etc. P. euphratica miR156j conservatively played a main role in plant development. Also germination rate and growth condition of 35S:MIR156j transgenic A. thaliana under salt treatment were better than that of the wild type, and the expression assay of gene SPL6, SPL9 and SPL11 demonstrated the down regulation caused by miR156 Based on the predicted stress associated target gene STRS2(stress response suppressor 2), we presumed that the molecular mechanism underlying the involvement of miR156 in salt tolerance may be ascribed to the regulation of STRS2 expression.
Keywords:Populus euphratica  overexpression  salt tolerance  Arabidopsis thaliana  miR156MicroRNAs (miRNAs)
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