绿色木霉纤维素酶AS3.3032固态发酵的研究

该研究以麦麸和汽爆蔗渣为主要原料 ,采用绿色木霉AS3 30 32 (Trichodermaviride)固态发酵生产纤维素酶 ,研究了氮源、碳源、表面活性剂、接种方式、培养基含水量、培养温度、培养基起始pH值对绿色木霉产酶活力的影响 .研究结果表明 :①以硫酸铵为氮源 ,其FPA ,CMC ,和 β Gase酶活力均较高 ,每克干曲分别高达 12 2 5FPAU g ,1470 0CMCU g和 119 3β GaseU g ;②碳源以麸蔗比为 3∶2时 ,FPA ,β Gase和CMC酶活力均为最高 ,每克干曲分别高达 138 2FPAU g ,134 6 β GaseU g和 16 0 3 1CMCU g ;③添加 0 1%的Tween 80和 0 5 %～ 0 7%的洗衣粉可分别提高FPA ,β Gase和CMC为 2 3倍、2 8倍、2 3倍和 3 1倍、3 7倍、3 0倍 ;④培养基含水量、培养温度、培养起始pH值分别为 2 5 0 % ,2 8℃和pH3 5 ,产酶活力最高

Production of cellulase by \%Trichoderma viride\% AS3.3032 in sloid state fermentation.

In the research, using wheat bran and steam exploded bagasse asmain substrate, cellulase is produced by solid state fermentation with Trichoderma viride AS3.3032. The effect on cellulase production is studied of nitrogen source, carbon source, surface active agent, inoculation, temperature, wate content and initial pH value of solid substrate. The results are:①With NH4SO4 as nitrogen source, FPA,CMC and β-gase all are high, 122.5 FPA U/g dried substrate, 1470.0 CMC U/g dried substrate and 119.3 β-Gase U/g dried substrate respectively;②With wheat bran and bigasse(their ratio 3 to 2) as carbon source, FPA, CMC and β-Gase get highest, 138.2 FPA U/g dried substrate, 134.6 β-Gase U/g dried substrate and 1603.1 CMC U/g dried substrate respectively;③Adding 0.1% Tween-80 to culture medium, FPA, β-Gase and CMC can increase by 230%,280% and 230% respectively, but with addition of 0.5%～0.7% washing powder, the three above indices increase by 310%,370%,300% resepctively;④The activity of produced cellulase goes up to the highest when the water amount of medium, the temperature and the initial pH are 250%,28℃ and 3.5.