| 京西葛组织培养及快速繁殖研究 |
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| 引用本文： | 张晓霞,丁友芳,史玲玲,张蕴薇,王富海,杨富裕,刘玉军.京西葛组织培养及快速繁殖研究[J].北京林业大学学报,2010,32(5):138-142. |
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| 作者姓名： | 张晓霞 丁友芳 史玲玲 张蕴薇 王富海 杨富裕 刘玉军 |
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| 作者单位： | 1北京林业大学林木育种国家工程实验室，林木花卉遗传育种教育部重点实验室，国家林业局树木花卉育种与生物工程重点开放实验室，北京林业大学生物科学与技术学院 2 中国农业大学动物科技学院3 北京三发植物种苗研究中心 |
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| 基金项目： | "十一五"国家科技支撵计划项目 |
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| 摘 要： | 通过京西葛茎段的离体培养和快速繁殖实验,探讨了京西葛不定芽的诱导、分化、增殖及生根的最佳培养条件。结果表明:以优良京西葛的具腋芽茎段为外植体,经2.2%NaClO灭菌25～30min后成活率可达50%;适于京西葛不定芽诱导的培养基为MS+NAA0.1mg/L+6-BA0.5mg/L,诱导分化率为88.89%;适于不定芽分化及继代培养的培养基为MS+NAA0.15mg/L+6-BA0.3mg/L+2,4-D0.2mg/L,继代芽分化率为83.33%,继代倍数达6～7倍;适于生根的培养基为1/2MS+NAA0.5mg/L,生根率达100%。本研究最终建立了京西葛的组织培养体系并初步建立起一套较为完善的快速繁殖技术体系。
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| 关 键 词： | 京西葛 茎段 组织培养 不定芽 愈伤组织 |
| 收稿时间： | 1900-01-01 |
Tissue culture and rapid propagation of Pueraria lobata |
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| ZHANG Xiao-xia,DING You-fang,SHI Ling-ling,ZHANG Yun-wei,WANG Fu-hai,YANG Fu-yu,LIU Yu-jun.Tissue culture and rapid propagation of Pueraria lobata[J].Journal of Beijing Forestry University,2010,32(5):138-142. |
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| Authors: | ZHANG Xiao-xia DING You-fang SHI Ling-ling ZHANG Yun-wei WANG Fu-hai YANG Fu-yu LIU Yu-jun |
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| Institution: | ZHANG Xiao-xia1,DING You-fang1,SHI Ling-ling1,ZHANG Yun-wei2,WANG Fu-hai3,YANG Fu-yu2,LIU Yu-jun1 1 National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,College of Biological Sciences and Biotechnology,Beijing Forestry University,100083,P.R.China,2 College of Animal Sciences and Technology,China Agricultural Unive... |
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| Abstract: | The optimal tissue culture conditions for induction,differentiation,proliferation and rooting of adventitious buds of Pueraria lobata Ohwi were studied through in vitro culture and rapid propagation technologies.The results showed that using stem segments with axillary buds from elite parent trees of P.lobata as explants,the survival rate could reach 50% after 25-30 min of sterilization with 2.2% NaClO.The most optimal medium for induction of adventitious buds was MS + NAA 0.1 mg/L + 6-BA 0.5 mg/L,and the i... |
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| Keywords: | Pueraria lobata stem segment tissue culture adventitious bud callus |
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