| 黑树莓的组织培养与快速繁殖 |
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| 引用本文： | 徐桂娟,罗晓芳,姚洪军.黑树莓的组织培养与快速繁殖[J].北京林业大学学报,2002,24(1):99-100. |
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| 作者姓名： | 徐桂娟 罗晓芳 姚洪军 |
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| 作者单位： | 北京林业大学良种繁育研究中心 |
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| 摘 要： | 为了提供市场所需的大量苗木,适应树莓规模化生产的需要,以黑树莓的带芽茎段为外植体进行组织培养试验.经试验对比筛选出黑树莓的最佳分化培养基为MS+6-BA 0.2mg/L+NAA 0.2mg/L+Sugar 20g/L+Agar 6 g/L,生根培养基为1/2 MS+IBA 0.2mg/L+Sugar 20?g/L+Agar 6g/L,生根率达100%.在两步移栽法中,移栽存活率分别为98%和100%.
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| 关 键 词： | 黑树莓 组织培养 快速繁殖 |
Tissue culture and rapid propagation of blackberry. |
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| Xu Guijuan,Luo Xiaofang ,Yao Hongjun.Research Centre of Improved Cultivar Breeding,Beijing For.Univ.,P.R.China..Tissue culture and rapid propagation of blackberry.[J].Journal of Beijing Forestry University,2002,24(1):99-100. |
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| Authors: | Xu Guijuan Luo Xiaofang Yao HongjunResearch Centre of Improved Cultivar Breeding Beijing ForUniv PRChina |
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| Institution: | Xu Guijuan,Luo Xiaofang ,Yao Hongjun.Research Centre of Improved Cultivar Breeding,Beijing For.Univ.,100083,P.R.China. |
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| Abstract: | Choosing stems with buds as explants, the authors succeeded in studing the best method for tissue culture and rapid propagation of blackberry . The surface sterilized explants were cultured on the medium MS + Vc 1.0 g/L+Sugar 20 g/L+Agar 10 g/L. MS+6-BA 0.2 mg/L+ NAA 0.2 mg/L+ Sugar 20 g/L+ Agar 6 g/L was the optimum medium for proliferation of bud clusters. Shoots were rootded on the medium 1/2 MS + IBA 0.2 mg/L+Sugar 20 g/L + Agar 6 g/L and the rate of rooting was 100%.Being acclimatized for 20 days, the rooted plantlets were transplanted to substrata in two successive steps, the survival rates of which were 98% and 100% respectively. |
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| Keywords: | blackberry tissue culture rapid propagation |
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