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| 柿树带化和刺槐丛枝的PCR检测 | |
| 引用本文： | 刘小勇,田素忠,秦国夫,沈瑞祥.柿树带化和刺槐丛枝的PCR检测[J].北京林业大学学报,1999,21(3):26-28. |
| 作者姓名： | 刘小勇 田素忠 秦国夫 沈瑞祥 |
| 作者单位： | 1. 中国科学院微生物研究所,100080,北京 2. 北京市林业局,100029,北京 3. 林业部森林病虫害防治总站,110034,沈阳 |
| 基金项目： | 国家自然科学基金;39370572; |
| 摘 要： | 根据植物菌原体１６ＳｒＲＮＡ基因序列，合成一对寡核苷酸引物，用ＳＤＳ ＣＴＡＢ改进法直接提取田间发病的柿树带化病、刺槐丛枝病树和健康树皮层的ＤＮＡ，用ＰＣＲ方法成功地从病树ＤＮＡ模板中扩增出约１．１７ｋｂ的特异产物，经酶切进一步证实为植物菌原体１６ＳｒＤＮＡ．此项研究表明用特异合成的引物Ｆ１６／Ｒ１６，经ＰＣＲ扩增可以快速灵敏地检测植物菌原体． |
| 关 键 词： | 柿树带化，刺槐丛枝，PCR检测 |
Examination of Persimmon Fasciation and Black Locust Witches' Broom with Polymerase Chain Reaction |
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| Liu Xiaoyong,Tian Suzhong,Qin Guofu,Shen Ruixiang.Examination of Persimmon Fasciation and Black Locust Witches'''' Broom with Polymerase Chain Reaction[J].Journal of Beijing Forestry University,1999,21(3):26-28. | |
| Authors: | Liu Xiaoyong Tian Suzhong Qin Guofu Shen Ruixiang |
| Institution: | Liu Xiaoyong1) Tian Suzhong2) Qin Guofu3) Shen Ruixiang1) |
| Abstract: | A pair of oligonucleotide primers was designed on the basis of Phytoplasmas 16SrRNA gene sequences. Total DNA was extracted from persimmon fasciation, black locust witches' broom and their healthy plant tissues collected from fields using amended SDSCTAB method. PCR products at the length of 1.17kb amplified from phytoplasmainfected tissues DNA were confirmed as Phytoplasmas 16SrDNA. This research indicated that Phytoplasmas could be inspected rapidly and sensitively by Polymerase Chain Reaction (PCR) with a specific primer pair F16/R16.\= |
| Keywords: | persimmon fasciation black locust witches'broom PCR examination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |