元宝枫组织培养研究

该文对元宝枫茎段组织培养中的外植体灭菌、褐变预防、增殖培养、根诱导等进行了试验研究.结果表明:元宝枫外植体最佳取材时期为4月份，由4年生和20年生母树上取材的外植体，经0.1％ HgCl2灭菌6 min后，污染率分别降为16.67％和14.29％；对于4龄和20龄母树上取材的外植体进行培养时，在培养基中分别添加500及750 mg/L PVP，可以较好地控制褐变；当MS培养基中附加0.01～0.05 μmol/L TDZ时，可有效地促进外植体增殖和生长；适宜的生根培养基为1/2MS+0.1 μmol/L IBA，每天光照16 h，生根率可达90.91％；幼苗经炼苗后盆栽，成活率在95％以上. 

Tissue culture of Acer truncatum.

The stem segments of Acer truncatum were used as explants to study sterilization techniques,browning prevention,proliferation and rooting in vitro. The results show that the optimum season for explant collection is April,the contamination rates of the explants from the 4 and 20 years old trees are 16.7％ and 14.3％ after the explants are sterilized in 0.1％ aqueous mercuric chloride for 6 min;for the explants from the 4 and 20 years old trees the addition of PVP of 500 mg/L and 750 mg/L in the medium can prevent browning effectively.The MS medium containing 0.01～0.05 μmol/L TDZ is most efficient in the proliferation of Acer truncatum.The ratio of rhizogenesis is 90.91％ in the optimum rooting medium of 1/2 MS medium supplemented with 0.1 μmol/L IBA and under 16 h of photoperiod;the survival ratio of transplanting is 95％ after training seedling.