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菊花茎叶外植体再生体系的研究
引用本文:高亦珂,丁国勋,张启翔,赵勃.菊花茎叶外植体再生体系的研究[J].北京林业大学学报,2001,23(1):32-33.
作者姓名:高亦珂  丁国勋  张启翔  赵勃
作者单位:1. 北京林业大学园林学院,100083,北京
2. 北京林业大学教育发展研究中心,100083,北京
基金项目:国家林业局“948”花卉高产栽培及花期调控技术项目资助(9840)
摘    要:以地被菊‘矮黄’、‘玉龙’和传统菊花‘金背大红’的叶盘和茎段为外植体进行再生培养 .实验结果表明 ,不同品种对相同激素水平的反应是不同的 :传统的大菊‘金背大红’的愈伤诱导率和分化率都远远低于两种地被菊 ;在相同条件下以茎段为外植体诱导愈伤率高于叶盘 ,但是叶盘的直接分化率高于茎段 .‘玉龙’和‘矮黄’的最适再生培养基组成为MS +NAA 0 .1mg/L + 6 BA 1mg/L和MS +NAA 0 .2mg/L + 6 BA 2mg/L ,‘金背大红’为MS +NAA 1mg/L + 6 BA 5mg/L

关 键 词:菊花  外植体  再生体系

Shoot regeneration from stem and leaf explant of Dendrathema grandiflorum
Gao Yike, Zhao Bo, Ding Guoxun, Zhang Qixiang. Journal of Beijing Forestry University.Shoot regeneration from stem and leaf explant of Dendrathema grandiflorum[J].Journal of Beijing Forestry University,2001,23(1):32-33.
Authors:Gao Yike  Zhao Bo  Ding Guoxun  Zhang Qixiang Journal of Beijing Forestry University
Abstract:Adventitious shoots were regenerated from leaf and stem explantsof three chrysanthemum cultivars. The optimum medium for both explant types in ‘Aihuang’ and ‘Yulong’ containd Murashige & Skoog basal medium supplemented with NAA 0.1mg/L+6-BA 1mg/L and NAA 0.2mg/L+6-BA 2mg/L, in ‘Jinbei Dahong’ contained Murashige & Skoog basal medium supplemented with NAA 1mg/L+6-BA 5mg/L. Generally, stem explants were superior to leaf explants. There were large cultivar difference in shoot regeneration frequency with three cultivars failing to respond over a wide range of hormone combination.
Keywords:Dendrathema grandiflorum    explants  shoot regeneration
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