不同温度和铝浓度对麻疯树生理指标的影响及外源钙的作用

以攀枝花地区麻疯树为材料，采用溶液培养方法，设置不同温度（24、29、34℃），研究不同浓度酸铝胁迫（Al 3＋的浓度为:对照、0.01、0.1、1、5 mmol/L）对3月龄麻疯树幼苗根系活力、叶片丙二醛（MDA）含量、叶片脯氨酸（Pro）含量、过氧化物酶（POD）活性等生理指标的影响. 结果表明:①相同Al3＋浓度处理下，随温度升高，幼苗根系活力降低，MDA、Pro含量增加，POD活性增加；②相同温度处理下，随Al3＋浓度增加，根系活力呈下降趋势，叶片MDA含量、Pro含量及POD活性呈上升趋势；③适度低温，适量的Al3＋（29℃，Al 3+浓度为0.01 mmol/L；24℃，Al 3+浓度为0.01、0.1 mmol/L）能增强根系活力，降低MDA和Pro含量，降低POD活性，有益于麻疯树幼苗的生长；④当Al3＋浓度增至1 mmol/L以上时，3个温度下处理的麻疯树幼苗都受到不同程度的伤害；⑤29℃、1 mmol/L Al 3+浓度下，增加溶液中Ca2＋浓度能减小酸铝对麻疯树幼苗的毒害作用.  

Changes of physiological indices of Jatropha curcas to aluminum stress under different temperatures and exogenous calcium

College of Life Science，Sichuan University，Chengdu, 610064，China.Jatropha curcas seedlings in the solutions culture were used to examine the effects of different concentrations of acid aluminum (Al³⁺：0, 0.01, 0.1, 1, 5 mmol/L)on the following physiological indices, including root vigor, malondialdehyde (MDA), proline (Pro) content and peroxidase(POD)activity of leaves at three temperatures (24, 29, 34℃). The results indicated that under the same Al^3＋concentration, the seedlings’root vigor decreased, the amount of MDA, Pro and the POD activity in leaves increased as the temperature rose. On the other hand, as the Al^3＋ concentration rose, root vigor trended to decrease, the amount of MDA and Pro and the POD activity trended to increase at each temperature. Mild amount of Al^3＋ stimulated the root vigor, brought down the MDA and Pro content as well as the POD activity at lower temperatures（29℃,Al³⁺:0.01 mmol/L；24℃,Al³⁺:0.01,01 mmol/L）. However, all seedlings at three temperatures were damaged more or less if the Al^3＋ concentration exceeded 1 mmol/L. Treated the seedlings with 1 mmol/L Al^3+ at 29℃, the damages decreased with the increase of exogenous calcium.