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地被菊直接体细胞胚发生研究
引用本文:蒋细旺,陈发菊,陆苗,蔡明,张启翔.地被菊直接体细胞胚发生研究[J].北京林业大学学报,2008,30(2):65-70.
作者姓名:蒋细旺  陈发菊  陆苗  蔡明  张启翔
作者单位:1 北京林业大学园林学院,国家花卉工程技术研究中心2 江汉大学生命科学学院3 三峡大学生物技术研究中心
基金项目:中国博士后科学基金项目(20060390404),北京市自然科学基金项目(6072019)
摘    要:该文建立了地被菊品种‘玉人面’直接体细胞胚受体体系。试验以‘玉人面’叶片为外植体,研究了培养基、生长调节剂等因子对其直接体细胞胚诱导的影响,同时还进行了抗生素敏感性试验。结果表明:①在相同浓度下,KT诱导体胚的效果优于6-BA;②体胚诱导培养基为MS+KT 20 mg/L+2,4-D 2.0 mg/L+NAA 0.5 mg/L,诱导10 d后,转入去除2,4-D的体胚发育培养基MS+KT 2.0 mg/L+NAA 0.5 mg/L中培养30 d,体胚发生率最高为93.0%,平均每外植体分化的体胚数为13.6个; ③MS基本培养基对‘玉人面’体胚发生的效果优于N 6、B5 培养基;④‘玉人面’直接体胚发生途径的基因转化的卡那霉素选择压为10 mg/L,头孢霉素前期(3~4周内)选择压为300 mg/L,后期(4周后)为100 mg/L。 

关 键 词:地被菊    直接体细胞胚发生    受体体系    抗生素
文章编号:1000-1522(2008)02-0065-06
收稿时间:2007-01-30
修稿时间:2007年1月30日

Direct somatic embryogenesis in ground-cover chrysanthemum
JIANG Xi-wang,CHEN Fa-ju,LU Miao,CAI Ming,ZHANG Qi-xiang.Direct somatic embryogenesis in ground-cover chrysanthemum[J].Journal of Beijing Forestry University,2008,30(2):65-70.
Authors:JIANG Xi-wang  CHEN Fa-ju  LU Miao  CAI Ming  ZHANG Qi-xiang
Institution:1 National Floriculture Engineering Research Center, College of Landscape Architecture, Beijing Forestry University, 100083, P. R. China; 2 College of Life Science, Jianghan University, Wuhan, 430056, P.R.China; 3 Biotechnology Research Center, Three Gorges University, Yichang City, Hubei Province, 443002, P. R. China.
Abstract:In this paper,acceptor system of direct somatic embryogenesis in ground-cover chrysanthemum cv.Yurenmian was established.The authors studied the effects of media,hormones on direct somatic embryogenesis through leaf segments as explants,and experiments of the sensitivity of antibiotics were also processed.Results showed that: 1) KT was more efficient than 2,4-D in induction somatic embryogenesis at the same concentration;2) somatic embryogenesis induction medium: MS KT 2.0 mg/L 2,4-D 2.0 mg/L NAA 0.5 mg/L.After induction 10 days,explants were transferred into somatic embryogenesis development medium,which was MS plus KT 2.0 mg/L and NAA 0.5 mg/L for 30 days.The highest rate of somatic embryogenesis reached 93.0%,average number of somatic embryogenesis per leaf segment explant was 13.6;3) MS basic medium was more efficient than N_6 and B_5 media for direct somatic embryogenesis;4) experiments of the sensitivity of antibiotics for gene transformation of direct somatic embryogenesis in ground-cover chrysanthemum cv.Yurenmian showed: the selection concentration of kanamycin was 10 mg/L,the selection concentration of cefotaxime was 300 mg/L in former period(during 3-4 weeks) and 100 mg/L later(after four weeks).
Keywords:ground-cover chrysanthemum  direct somatic embryogenesis  acceptor system  antibiotics
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