| 毛白杨TIR-NBS-LRR基因转化烟草的研究 |
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| 引用本文： | 李海霞,张志毅,张谦,安新民,李琰,刘婷婷.毛白杨TIR-NBS-LRR基因转化烟草的研究[J].北京林业大学学报,2009,31(1):73-78. |
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| 作者姓名： | 李海霞 张志毅 张谦 安新民 李琰 刘婷婷 |
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| 作者单位： | 北京林业大学林木育种国家工程实验室，林木花卉遗传育种教育部重点实验室 |
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| 基金项目： | 国家自然科学基金，高等学校博士学科点专项科研基金 |
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| 摘 要： | 该文采用农杆菌介导法将从毛白杨中克隆得到的TIR-NBS-LRR抗病基因（PtDRG01）导入烟草中，经抗生素筛选和PCR分子检测，获得了一批阳性转化植株。进而对这些阳性转化植株进行烟草花叶病毒接种实验，从表型观察结果发现，在接种病毒1周和6周后，转基因烟草株系TG-11的发病程度明显低于非转基因烟草。荧光定量分析结果显示，转基因烟草株系TG-11叶片中的烟草花叶病毒(TMV)数量显著低于非转基因植株，且该转基因株系中的PtDRG01基因转录水平显著高于非转基因植株。这些结果表明，毛白杨PtDRG01基因具有明显的抗病功能，其高效表达能够显著提高烟草的抗TMV能力。该文通过对烟草的遗传转化与病毒接种实验，鉴定了PtDRG01基因的功能，可为其他TIR-NBS-LRR基因的功能鉴定提供参考。
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| 关 键 词： | TIR-NBS-LRR基因 转基因烟草 烟草花叶病毒 抗病性 |
| 收稿时间： | 1900-01-01 |
Transformation of tobaccos with a TIR-NBS-LRR gene isolated from Populus tomentosa Carr |
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| LI Hai-xia,ZHANG Zhi-yi,ZHANG Qian,AN Xin-min,LI Yan,LIU Ting-ting.Transformation of tobaccos with a TIR-NBS-LRR gene isolated from Populus tomentosa Carr[J].Journal of Beijing Forestry University,2009,31(1):73-78. |
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| Authors: | LI Hai-xia ZHANG Zhi-yi ZHANG Qian AN Xin-min LI Yan LIU Ting-ting |
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| Institution: | National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, 100083, P. R. China. |
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| Abstract: | A TIR-NBS-LRR gene(PtDRG01)isolated from Populus tomentosa Carr.was transferred into tobacco's via the Agrobacterium-mediated method.Antibiotics screening and PCR analysis showed that the gene of interest was integrated into tobacco genome.Resistance test with tobacco mosaic virus(TMV) suggested that the disease severity of transgenic tobacco TG-11 was significantly lower than that of non-transgenic tobaccos one week and six weeks after inoculation.By real-time PCR,the authors found that the number of virus... |
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| Keywords: | Populus tomentosa Carr TIR-NBS-LRR type of gene transgenic tobacco tobacco mosaic virus disease resistance |
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