| 粉红粘帚霉67—1厚垣孢子形成与贮存研究 |
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| 引用本文： | 董佩佩,康涛,孙漫红,李世东,罗明.粉红粘帚霉67—1厚垣孢子形成与贮存研究[J].黑龙江农业科学,2013(10):48-51. |
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| 作者姓名： | 董佩佩 康涛 孙漫红 李世东 罗明 |
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| 作者单位： | [1]新疆农业大学农学院,新疆乌鲁木齐830052 [2]中国农业科学院植物保护研究所／农业部作物有害生物综合治理综合性重点实验室,北京100081 |
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| 基金项目： | 国家“863”计划资助项目（2011AA10A205）;国家“十二五”科技支撑计划资助项目（2012BAD19801）;行业技术体系资助项目（Nycytx-35-gw29） |
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| 摘 要： | 为研制粘帚霉生防剞剂，以粉红粘帚霉67—1为材料，对粉红粘帚霉67—1厚垣孢子的生长过程、孢子结构、贮存介质和制剂含水量以及厚垣孢子和分生孢子的货架期比较进行了研究。结果表明：在特定的培养基中，粉红粘帚霉67—1在培养24h后产生厚垣孢子，厚垣抱子形成于茵丝顶端或中部，并可以脱落游离到培养基中。电镜下可以看到圆形或椭圆形厚垣孢子以及加厚的细胞壁。相同贮存条件下，厚垣孢子的货架期比分生孢子长。低温条件下，10％含水量的贮存介质更利于厚垣孢子的贮存。
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| 关 键 词： | 粉红粘帚霉 厚垣孢子 货架期 生物防治 |
Formation and Storage of Clonostachys rosea 6 7-1 Chlamydospores |
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| DONG Pei-pe,KANG Tao,SUN Man-hong,LI Shi-dong,LUO Ming.Formation and Storage of Clonostachys rosea 6 7-1 Chlamydospores[J].Heilongjiang Agricultural Science,2013(10):48-51. |
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| Authors: | DONG Pei-pe KANG Tao SUN Man-hong LI Shi-dong LUO Ming |
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| Institution: | 1. College of Agronomy, Xinjiang Agricultural University, Urumqi, Xinjiang 830052 ; 2. Key Labo- ratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protec- tion, Chinese Academy of Agricultural Sciences, Beijing 100081) |
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| Abstract: | In order to develop Gliocladium biocontrol preparation, taking Clonostachys rosea 67-1 chlamydospores as test materials, the growth process, morphology, shelf life, storage media, water content and comparation between chla- mydospores and conidia were studied. The results showed that chlamydospores produced in specific medium after cul- turing 24 hours,chlamydospores formed in the centre of hyphae or at the top,and it could fall off into the medium. Circular or ellipse thick wall structure and incrassate cell wall could be seen under the electron microscope. In the same storage conditions,chlamydospores had longer shelf life than condia. Under low temperature, the storage medium of 10% water content could extend shelf life of chlamydospores of Clonostachys rosea 67-1. |
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| Keywords: | Clonostachys rosea chlamydospores shelf life biological control |
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