| 金正日球根海棠叶片组织培养诱导的研究 |
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| 引用本文： | 张凤生,樊绍翥,薛丹丹,姜淼,李健虹,王保菊,武斌,赵娜.金正日球根海棠叶片组织培养诱导的研究[J].黑龙江农业科学,2009(2):17-19. |
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| 作者姓名： | 张凤生 樊绍翥 薛丹丹 姜淼 李健虹 王保菊 武斌 赵娜 |
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| 作者单位： | 哈尔滨市农业科学院,黑龙江哈尔滨,150070 |
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| 摘 要： | 以金正日球根海棠（Begonia tuberhybrieda Vosa）的叶片为外植体进行组织培养的诱导，以Ms培养基为基本培养基，并附加不同浓度的细胞分裂素6-BA以及生长素NAA或2，4-D进行组织培养和植株再生。经实验筛选出最适合诱导愈伤组织的培养基是Ms＋6-BA1．0～2．0＋NAA0．1～0．5；脱分化的最佳激素组合是培养基Ms＋6-BA1．0＋NAA0．5。
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| 关 键 词： | 球根海棠 组织培养 诱导 |
Study on Leaves of Kim Jong-il Begonia tuberhybrieda Vosa Induced by Tissue Culture |
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| ZHANG Feng-sheng,FAN Shao-zhu,XUE Dan-dan,JIANG Miao,LI Jian-hong,WANG Bao-ju,WU Bin,ZHAO Na.Study on Leaves of Kim Jong-il Begonia tuberhybrieda Vosa Induced by Tissue Culture[J].Heilongjiang Agricultural Science,2009(2):17-19. |
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| Authors: | ZHANG Feng-sheng FAN Shao-zhu XUE Dan-dan JIANG Miao LI Jian-hong WANG Bao-ju WU Bin ZHAO Na |
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| Institution: | Harbin Academy of Agricultural Sciences;Harbin;Heilongjiang 150070 |
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| Abstract: | Leaves of Kim Jong-il Begonia tuberhybrieda Vosa were used as explants to conduct tissue culture induction.Ms medium was basic medium,adding with different concentrations of cytokinin 6-BA and NAA or 2,4-D to carry on plant tissue culture and regeneration.The experiment selected the most suitable callus induction of the medium was MS+BA1.0~2.0+NAA0.1~0.5;dedifferentiation hormone combination was the best medium MS+BA1.0+NAA0.5. |
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| Keywords: | Begonia tuberhybrieda Vosa tissue culture induction |
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