| 优化水稻培养条件测定其果胶酶活力及灰度变化 |
| |
| 引用本文： | 王旭达,丰明,李景鹏.优化水稻培养条件测定其果胶酶活力及灰度变化[J].黑龙江农业科学,2010(3):5-8. |
| |
| 作者姓名： | 王旭达 丰明 李景鹏 |
| |
| 作者单位： | 辽宁省农业科学院,大连生物技术研究所,辽宁,大连,116024 |
| |
| 摘 要： | 采用浸泡发芽培养方法改变水稻分子内部构象,测定其变性淀粉果胶酶活力的变化,选择最佳浸泡发芽条件并测定变性后淀粉灰度。结果表明:最佳培养条件为浸泡温度25℃,浸泡方式为浸泡4 h断水10 h,加碱量为0.03%Ca(OH)2,培养时间为2.5 d,在25℃条件下发芽6 d,变性淀粉果胶酶活性最高,灰度较好。从而成功实现对发芽水稻变性淀粉果胶酶活性及其灰度分布的测定。
|
| 关 键 词： | 发芽水稻 变性淀粉 果胶酶活力 灰度 |
Optimize the Condition of Rice Cultivation and Determinate the Change of the Pectinase Activity and the Gray-scale |
| |
| WANG Xu-da,FENG Ming,LI Jing-peng.Optimize the Condition of Rice Cultivation and Determinate the Change of the Pectinase Activity and the Gray-scale[J].Heilongjiang Agricultural Science,2010(3):5-8. |
| |
| Authors: | WANG Xu-da FENG Ming LI Jing-peng |
| |
| Institution: | (Dalian Biotechnology Institute of Liaoning Academy of Agricultural Sciences,Dalian, Liaoning 116024) |
| |
| Abstract: | By soaking the rice for the cultivation the molecular conformation changed,and determinated the change of the pectinase activity,then determinated the best immersion and germination conditions and the gray-scale.The results showed that the best conditions for cultivate was that immersion temperature is 25℃,immersion mode is 4 hours immersion and 10 hours un-immersion,immersion alkali content is 0.03% Ca(OH)2, immersion time is 2.5 days, germination temperature is 25℃,germination time is 6 days, the pectinase activity and the gray-scale of the modified starch were best at this time.The pectinase activity and the gray-scale of rice modified starch were determinated successfully. |
| |
| Keywords: | germination rice modified starch activity of pectinase gray-scale |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
