| 意蜂三型蜂间哺育王浆蛋白组分差异的研究 |
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| 引用本文： | 刘祥伟.意蜂三型蜂间哺育王浆蛋白组分差异的研究[J].黑龙江农业科学,2012(1):60-63. |
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| 作者姓名： | 刘祥伟 |
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| 作者单位： | 黑龙江省农业科学院牡丹江分院 |
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| 基金项目： | 现代农业产业技术体系(蜜蜂)资助项目(CARS-45-SYZ5) |
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| 摘 要： | 为研究蜜蜂三型蜂幼虫期间食物的差异,现对意大利蜜蜂三型蜂间哺育王浆蛋白组分的差异性进行研究。对工蜂幼虫哺育浆、雄蜂幼虫哺育浆、蜂王幼虫哺育浆分别用水和磷酸缓冲液进行提取,利用聚丙烯酰胺凝胶电泳分析。结果表明:工蜂和雄蜂各日龄幼虫哺育浆间存在不同程度的差异;各个日龄蜂王幼虫哺育浆的蛋白质谱带基本相同;哺育浆分别经水和磷酸缓冲液提取,结果存在差异。
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| 关 键 词： | 意大利蜜蜂 蜂王浆 聚丙烯酰胺凝胶电泳 蛋白质 |
Difference in Protein Composition of Breeding Jelly in Apis mellifera ligustica |
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| LIU Xiang-wei.Difference in Protein Composition of Breeding Jelly in Apis mellifera ligustica[J].Heilongjiang Agricultural Science,2012(1):60-63. |
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| Authors: | LIU Xiang-wei |
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| Institution: | LIU Xiang-wei (Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang,Heilongjiang 157041) |
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| Abstract: | In order to study the food difference between three types of bee during larva period,the difference of royal jelly protein component bred by three types of Apis mellifera ligustica were studied.The protein component of breeding jelly extracted by water and PBS,respectively from the worker,drone and queen of were analyzed by SDS-PAGE.The results showed that there were differences in protein components of jelly bred by various instars of worker and drone.The protein bands were no difference in jelly fed by various instars of queen.The protein bands were different in jelly extracted by water and PBS. |
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| Keywords: | Apis mellifera ligustica royal jelly SDS-PAGE protein composition |
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