48份玉米自交系的SSR遗传多样性分析

利用SSR标记对48个黑龙江省玉米自交系进行遗传多样性分析,初步进行了杂种优势群划分。从100对SSR引物中筛选取20对扩增带清晰且具有稳定多态性的引物。结果表明:20对引物在供试材料中共检测出105个等位基因变异,平均每对引物检测到等位基因数位5.25个,变化范围在3~7个,多态性信息含量变化范围为0.405~0.793,平均多态性信息量为0.664。48个自交系之间的遗传相似系数变化范围为0.400~0.905。UPGMA聚类分析结果表明:供试自交系被划分为5大类群,聚类结果与系谱分析基本一致。因此,SSR标记可以进行玉米自交系遗传变异分析,并用于杂种优势群的划分。

Analysis of Genetic Diversity of 48 Local Maize Inbred Lines by SSR Markers

The genetic diversity of 48 maize inbred lines in Heilongjiang was analyzed by SSRs and the heterotic groups were classified. 20 pairs of primers whose bands were clearly amplified and discrepant were selected out from 100 pairs of primers. Totally 105 alleles were identified, and the average number of alleles per SSR locus was 5.25 in these lines with a range from 3 to 7. Polymorphism information content （PIC） values varied from 0. 405 to 0. 793 with an average of 0. 664. The genetic similarity coefficient （GS） ranged from 0. 400 to 0. 905 among 48 inbred lines. By clustering analysis, 48 inbred lines were divided into 5 heterotic groups. Cluster analysis by UPGMA were basically corresponding to their pedigree analysis. Therefore, SSR could be used to di vide beterotie groups.