| 侧孢芽孢杆菌HBL-26发酵条件的优化研究 |
| |
| 引用本文： | 赵云彤.侧孢芽孢杆菌HBL-26发酵条件的优化研究[J].黑龙江农业科学,2012(5):112-116. |
| |
| 作者姓名： | 赵云彤 |
| |
| 作者单位： | 黑龙江省农业科学院牡丹江分院,黑龙江牡丹江,157041 |
| |
| 摘 要： | 为对拮抗菌株HBL-26的发酵培养基进行优化,通过正交试验设计对发酵液的4个单因子进行优化筛选,经软件分析后确定发酵培养基最佳配方为:葡萄糖6g.L-1、牛肉膏14g.L-1、NaCl 6g.L-1、MnSO40.05g.L-1。同时,得到回归方程:Y=5.34 X1-1.72 X2+14.44 X3-6.43 X4+8.35,其标准差S=14.23;F=4.202;显著值Sig.=0.019<0.05。最后通过单因子试验对菌株HBL-B26的发酵条件进行了优化,确定的最优培养条件为:培养基初始pH 8、发酵温度30℃、发酵最佳时间为48h。
|
| 关 键 词： | 侧孢芽孢杆菌 发酵条件 优化 |
Optimization of Fermentation Condition of Bacillus laterosporus HBL-26 |
| |
| ZHAO Yun-tong.Optimization of Fermentation Condition of Bacillus laterosporus HBL-26[J].Heilongjiang Agricultural Science,2012(5):112-116. |
| |
| Authors: | ZHAO Yun-tong |
| |
| Institution: | ZHAO Yun-tong(Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang,Heilongjiang 157041) |
| |
| Abstract: | In order to optimize the fermentation culture medium of HBL-26,four single factors were selected by the orthogonal design.The optimum medium was glucose 6g.L-1,beef extract14g.L-1,NaCl 6g.L-1 and MnSO 4 0.05g.L-1.The regression model was obtained Y=5.34 X 1-1.72 X 2 +14.44 X 3-6.43 X 4 +8.35,S= 14.23;F=4.202;Sig=0.019<0.05.Four factors were tested to increase the antibiotics yield by single-factor test method.The result showed that the optimum process parameters were initial pH was 8.0,culture temperature was 30℃and the best fermentation time was 48h. |
| |
| Keywords: | Bacillus laterosporus fermentation condition optimization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
