沙棘叶水溶性多糖分级组分抗氧化活性的研究

为了研究沙棘叶水溶性多糖分级组分的体外抗氧化活性,采用热水浸提、乙醇分级沉淀,得到沙棘叶水溶性多糖分级组分：WPFH60、WPFH70、WPFH80,经Sevag法脱蛋白,透析,冷冻干燥后备用。通过还原力、清除超氧阴离子、清除羟基自由基和抑制H2O2诱导红细胞氧化溶血试验来评价3个组分的体外抗氧化能力,并与BTH进行比较。结果表明：WPFH60、WPFH70和WPFH80均具有一定的抗氧化活性,且呈显著的量效关系。其中,WPFH70对O2-和OH-具有较强的清除作用,IC50分别为：311.1和179.9μg.mL-1;对H2O2诱导红细胞氧化溶血及MDA生成有很强的抑制作用,IC50分别为：194.3和174.5μg.mL-1。WPFH60、WPFH70和WPFH80在一定浓度范围内都具有抗氧化性,是一种天然的抗氧化剂。

Study on the Antioxidation Activities of Rating Water Soluble Polysaccharides by Alcohol from Foliage of Hippophae Rhamnoides L.

In order to study the antioxidant activities of rating water soluble polysaccharides by alcohol from foliage of Hippophae Rhamnoides L.（WPFH）,the water soluble polysaccharides were extracted from foliage of Hippophae rhamnoides L.by hot water and purified by fractional precipitation with ethanol（60%,70%,80%）.The fractional precipitation was named WPFH60,WPFH70 and WPFH80,respectively.They were deproteinized by Sevag method,then dialyzed and freeze drying.The antioxidant effects of WPFH60,WPFH70 and WPFH80 were evaluated in vitro by four different experiments：measuring reducing power,eliminating superoxide anion free radical experiment,eliminating hydroxyl free radical experiment,inhibiting mice erythrocyte hemolysis and lipid peroxidation MDA induced by H2O2experiment.The results showed that the data obtained in the in vitro models clearly established the antioxidant potency of the WPFH60,WPFH70 and WPFH80 in a dose dependent manner,among which,the WPFH70 was found to be most strongly scavenge the superoxide anion free radical and hydroxyl free radical,where the IC50 value of WPFH70 was found to be 311.1 and 179.9 μg·mL-1 respectively.The WPFH was found to be most strongly inhibit the mice erythrocyte hemolysis and lipid peroxidation MDA induced by H2O2 experiment,where the IC50 value of WPFH70 was found to be 194.3 and 174.5 μg·mL-1 respectively.The WPFH60,WPFH70 and WPFH80 could be a natural antioxidant.