| DGGE技术在土壤微生物群落研究中的应用 |
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| 引用本文： | 宋洋,李柱刚.DGGE技术在土壤微生物群落研究中的应用[J].黑龙江农业科学,2010(7):5-8. |
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| 作者姓名： | 宋洋 李柱刚 |
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| 作者单位： | 黑龙江省农业科学院,生物技术研究所/黑龙江省作物与家畜分子育种重点实验室,黑龙江,哈尔滨,150086 |
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| 基金项目： | 黑龙江省科技厅国际合作重点资助项目 |
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| 摘 要： | 现代分子生物学的变性梯度凝胶电泳(Denaturing Gradient Gel Electrqphoresis,DGGE)技术是土壤微生物群落研究的有效工具,它直接分离土壤微生物的DNA片段,比传统的培养方法更具明显的优势,已成为研究土壤微生物的重要手段之一。对DGGE技术的原理及其在土壤微生物群落研究中的应用情况、优点和局限性进行了介绍。
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| 关 键 词： | DGGE 土壤微生物 群落结构 |
Application of DGGE in Soil Microorganism |
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| SONG Yang,LI Zhu-gang.Application of DGGE in Soil Microorganism[J].Heilongjiang Agricultural Science,2010(7):5-8. |
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| Authors: | SONG Yang LI Zhu-gang |
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| Institution: | (Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang,Harbin,Heilongjiang 150086) |
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| Abstract: | Based on modern molecular technology,DGGE is a powerful tool for the analysis to understand community structure of soil microorganism,which can directly separating DNA clips.It has obvious advantages and it is one of the most useful research methods in soil microorganism.In this paper,the principle and the application of DGGE in soil microorganism were briefly introduced,while the advantages and limitations of this technique were also evaluated. |
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| Keywords: | DGGE |
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