水稻粒形性状的QTL分析

为了促进水稻粒形性状分子标记辅助选择育种研究,以籼稻二九南和粳稻龙稻5号杂交衍生的重组自交系群体为材料,对粒长、粒宽和粒厚3个粒形性状进行数量性状基因定位(Quantitative trait loci,QTL)分析。结果表明:采用完备区间作图法(ICIM),共检测到7个控制粒形性状的QTL,包括3个粒宽QTL,4个粒厚QTL,它们分布于第2、3、5、11和12号染色体上,其中4个主效QTL包括1个控制粒宽的qGW5a和3个控制粒厚的qGT2a、qGT11a以及qGT12a,但未检测到控制粒长的QTL位点。进一步分析表明,3个控制粒宽性状的QTL能解释28.44%的表型贡献率,单个表型贡献率为6.61%~13.81%;而4个控制粒厚性状的QTL能解释17.53%的表型贡献率,单个表型贡献率为7.32%~15.30%。

QTL Analysis on Grain Shape of Rice

In order to promote molecular genetic improvement of rice grain shape traits to assist breeding research, recombinant inbred lines（RILs）derived from a cross between Longdao 5 of Japonica and Erjiunan of in- dica were used to map QTL controlling grain shape traits. Based on the Inclusive Composite Interval Mapping （ICIM） ,a total of 7 QTLs were detected including 3 QTLs control for grain width and 4 QTLs control for grain thickness,which were distributed on chromosomes 2,3,5,11 and 12.4 major QTLs included 1 QTL qGWSa for grain width and 3 QTLs qGT2α,qGT11α and qGT12α for grain thickness. But it detected no one to control grain length. Further analysis showed that 3 QTL for grain width could explain 28.44 % of phenotypic variance respectively and the contribution of single QTL was between 6.61%- 13.81%. 4 QTLs for grain thickness could explain 17.53 % of phenotypic variance respectively and the contribution of single QTL was between 7.32-15.30%.