| 砂生槐单粒种子DNA的快速提取及RAPD检测 |
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| 引用本文： | 石梦菲,拉多,张勇群.砂生槐单粒种子DNA的快速提取及RAPD检测[J].黑龙江农业科学,2014(8):35-38. |
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| 作者姓名： | 石梦菲 拉多 张勇群 |
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| 作者单位： | 西藏大学理学院;西藏自治区高等学校重点实验室生物技术实验室; |
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| 摘 要： | 为探讨快速提取种子DNA的有效方法,以砂生槐单粒种子为试验材料,在传统CTAB法基础上建立一种快速提取DNA的方法,并通过紫外分光光度法、凝胶电泳和RAPD-PCR检测所提基因组DNA。结果表明:快速法提取得到了质量较好、浓度及纯度较高的DNA,可以满足分子生物学实验的要求。此方法操作方便、快捷,是一种有效的砂生槐种子的DNA提取方法。
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| 关 键 词： | 砂生槐 单粒种子 DNA快速提取 RAPD检测 |
Rapid DNA Isolation and RAPD Detection of Single Seed from Sophora moorcroftiana |
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| Institution: | SHI Meng-fei , LA-Duo , ZHANG Yong-qun (1. School of Science, Tibet University, Lhasa, Tibet 850000; 2. Key Laboratory of High School in Tibet Autonomous Region,Biotechnology Laboratory, Lhasa, Tibet 850000) |
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| Abstract: | In order to explore the effective extraction of seeds DNA rapidly, taking single seed of Sophora rnoorcroftiana as materials, a method for rapid isolation of genomic DNA from single seed of Sophora moorcroftiana was established based on the traditional CTAB method. The extracted DNA was assessed by ultraviolet photometer, agarose gel electrophoresis and RAPD-PCR, respectively. The results showed that the DNA extracted by rapid method was high output and purity, the quality of genomic DNA could extensively meet the requirements for many molecular biology experiments. It is a rapid, simple and effective method for ex- tracting genomic DNA from single seed of Sophora rnoorcroftiana. |
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| Keywords: | Sophora rnoorcroftiana single seed rapid DNA isolation RAPD detection |
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