龙麦20小麦品种17+18与7+8亚基近等基因系间品质差异的初步研究

为了解小麦Glu-B1位点HMW-GS 17＋18与7＋8的遗传差异，利用生化标记和连续6次选择性回交的方法将17＋18亚基转移到黑龙江省小麦品种龙麦20中，获得了龙麦20的Glu-B1位点HMW-GS17＋18与7＋8的近等基因系（NILs）。2006年将NILs种植在黑龙江省农业科学院育种研究所的试验田，田间设计采用双列对比排列，4次重复。近等基因系品质分析结果表明，17＋18亚基类型与7＋8亚基类型相比，面筋指数、沉降值、形成时间、稳定时间和断裂时闽等重要的品质参数均有一定程度的提高。在5＋10亚基遗传背景下，Glu-B1位点17＋18亚基对面筋强度仍有一定影响，因此在选育强筋小麦时，17＋18应作为优质亚基予以考虑。

Study on the Quality Difference of NILs with HMW-GS 17+18 and 7+8 in Longmai 20

In order to determine the genetic differences between high molecular weigh glutenin s ubunits （HMW-GS） 17 ＋18 and 7＋8, H MW-GS 17＋18 was introduced into Longmai 20 by 6 consecutive backcrosses with biochemical marker assisted selection. The near isogenie lines （NILs） of HMW-GS 17＋ 18 and 7＋8 were obtained and grown in the experimental field of Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences in 2006. The field experiments were designed using the method of two line-contrast arrangement with four replicates. The result of the NILs showed as below,as compared to NILs with subunit 7＋8,the NILs with subunit 17＋18 were higher in gluten index, Zeleny sedimentation, development time, stability, breakdown time, respectively. The impact of the HMW-GS 17＋18 on gluten strength was positive in NILs containing HMW-GS 5＋ 10,suggesting that HMW-GS 17＋18 could be a good subunit for breeding strong gluten wheat.