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| 柱层析法对菌株snef8有效成分的初步分离 | |
| 引用本文： | 谭可菲,段玉玺,朱晓峰,陈井生,罗宝君,赵秀梅,王连霞.柱层析法对菌株snef8有效成分的初步分离[J].黑龙江农业科学,2011(4):64-66. |
| 作者姓名： | 谭可菲 段玉玺 朱晓峰 陈井生 罗宝君 赵秀梅 王连霞 |
| 作者单位： | 1. 黑龙江省农业科学院,齐齐哈尔分院,黑龙江,齐齐哈尔,161006;沈阳农业大学,植物保护学院,辽宁,沈阳,110161 2. 沈阳农业大学,植物保护学院,辽宁,沈阳,110161 3. 黑龙江省农业科学院,大庆分院,黑龙江,大庆,163316 4. 黑龙江省农业科学院,齐齐哈尔分院,黑龙江,齐齐哈尔,161006 |
| 基金项目： | "十一五"国家科技支撑计划资助项目 |
| 摘 要： | 采用硅胶柱层析的方法,对snef8菌株中的杀线虫活性物质进行了初步的分离,生物法测定各组份的杀线虫活性,减压浓缩有活性的组份制备粗提液.结果表明:菌液中的杀线虫活性物质主要集中于第8～第12号管中,其杀线虫活性接近于100%.此法不但可以除去部分杂质,提高粗提液的纯度,还可以应用制备型硅胶柱,大量制备富集粗提液,将其作... |
| 关 键 词： | shef8真菌 南方根结线虫 杀线虫活性 生物测定 硅胶柱层析法 |
Primary Separation of the Activity Substances of snef 8 against Meloidogyne incognita by Chromatography |
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| TAN Ke-fei,DUAN Yu-xi,ZHU Xiao-feng,CHEN Jing-sheng,LUO Bao-jun,ZHAO Xiu-mei,WANG Lian-xia.Primary Separation of the Activity Substances of snef 8 against Meloidogyne incognita by Chromatography[J].Heilongjiang Agricultural Science,2011(4):64-66. | |
| Authors: | TAN Ke-fei DUAN Yu-xi ZHU Xiao-feng CHEN Jing-sheng LUO Bao-jun ZHAO Xiu-mei WANG Lian-xia |
| Institution: | 1.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences;Qiqihar;Heilongjiang 161006;2.Plant Protection College of Shenyang Agricultural University,Shenyang,Liaoning 110161;3.Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing,Heilongjiang 163316) |
| Abstract: | The preliminary separation of the activity substances from the Fermentation against Meloidogyne incognitawas investigated by using silica gel column chromatography.The activity against Meloidogyne incognitaof each component was tested by vivo measurement.The crude extracts was prepared by decompressed and concentrated the activity component.The result showed that the activity substances against Meloiogyne incognita was in the bottles from No.8 to No.12.The methods could not only remove some impurities,improve the purity of the crude extracts,but also enrich lots of crude extracts by using the preparative silica gel column chromatography.This crude extracts could be used in agricultural production directly and the control effects was better. |
| Keywords: | snef8 fungus Meloidogyne spp nematicidal activity bio-assay silica-chromatography |
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