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春小麦空间诱变后代的变异研究
引用本文:郭怡璠,张宏纪,刘文林,孙岩,刘东军,耿宏伟,杨淑萍.春小麦空间诱变后代的变异研究[J].黑龙江农业科学,2014(10):1-4.
作者姓名:郭怡璠  张宏纪  刘文林  孙岩  刘东军  耿宏伟  杨淑萍
作者单位:1. 黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨,150086
2. 黑龙江省农业科学院脱毒苗木研究所,黑龙江哈尔滨,150086
基金项目:国家“863”航天育种资助项目
摘    要:为了进一步探索和研究春小麦空间诱变后代的变异,对经返地卫星搭载纯系小麦龙麦26和克丰10号航天诱变种子种植后选育出的7份品系的农艺性状进行了调查,同时对其DNA多态性进行了分析。结果表明:变异品系较原品种在农艺性状上均有所变异,但差异不显著。变异品系与亲本之间存在着不同程度的微卫星多态性,在有效扩增的27对引物中出现3种突变类型:扩增片段增多、扩增片段减少和扩增片段长度有差异,出现的单一突变类型居多。

关 键 词:小麦  空间诱变  性状变异  微卫星标记

Study on Spring Wheat Variation of Progeny by Space Induced Mutation
GUO Yi-fan,ZHANG Hong-ji,LIU Wen-lin,SUN Yan,LIU Dong-jun,GENG Hong-wei,YANG Shu-ping.Study on Spring Wheat Variation of Progeny by Space Induced Mutation[J].Heilongjiang Agricultural Science,2014(10):1-4.
Authors:GUO Yi-fan  ZHANG Hong-ji  LIU Wen-lin  SUN Yan  LIU Dong-jun  GENG Hong-wei  YANG Shu-ping
Institution:GUO Yi-fan ,ZHANG Hong-ji ,LIU Wen-lin ,SUN Yan ,LIU Dong-jun ,GENG Hong-wei, YANG Shu-ping (1. Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Hei- longjiang 150086 ;2. Virus-free Seeding Research Institute of Heilongjiang Academy of Agri- cultural Sciences, Harbin, Heilongjiang 150086)
Abstract:In order to explore and study the mutation of spring wheat offspring further, the agronomic trait and DNA polymorphism of 7 lines after the planting of space mutation seed of pure line I.ongmai 26 and Kefeng 10 were investigated and analyzed. The results showed that the mutant plants had more variation in yield charac- ters than the original varieties and mostly positive mutation. Mutant plants and native species existed between different levels of microsatellite polymorphism,there were 3 types of mutations amplified among 27 groups of SSR primers:fragments amplification increased,amplified fragment decreased and different length of amplified fragment,the single mutation types were in the majority.
Keywords:wheat  space induction  character variance  microsatellite analysis
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