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| 中国家驴品种遗传多样性及母系起源研究 | |
| 引用本文： | 王全喜,陈建兴,闵令江,乌日图那生,乌云.中国家驴品种遗传多样性及母系起源研究[J].内蒙古农业大学学报(自然科学版),2012,33(2):7-11. |
| 作者姓名： | 王全喜 陈建兴 闵令江 乌日图那生 乌云 |
| 作者单位： | 1. 内蒙古农业大学研究生院,呼和浩特,010018 2. 内蒙古农业大学动物科学学院,呼和浩特,010018 3. 青岛农业大学动物科技学院,青岛,266109 4. 阿拉善盟阿拉善左旗巴润别立动物卫生监督站,阿拉善左旗,750300 5. 赤峰市林西县农牧业局,赤峰,025250 |
| 基金项目： | 青岛市科技计划基础研究项目 |
| 摘 要： | 为了给中国家驴遗传资源的评价、保护和利用提供一些分子生物学基础,本研究通过自行设计引物,采用PCR-SSCP技术对中国家驴线粒体DNA( Mitochondrial DNA,mtDNA)细胞色素b(Cytochrome b,Cytb)基因308bp片段序列的变异进行检测来探讨其遗传多样性和母系起源.发现了3种基因型,结合GenBank已公布的数据进行分析发现在中国家驴品种中共存在15个突变位点,17个单倍型,通过计算各群体的遗传多样性指数发现中国家驴的遗传多样性比较丰富.另外,还通过与两种非洲野驴的Cytb序列进行比对和网络聚类分析方法对所有家驴的样品进行了母系起源的研究,证明家驴起源于非洲的索马里驴和努比亚驴. |
| 关 键 词： | 驴 Cytb 遗传多样性 母系起源 |
GENETIC CIVERSITH AND MATERNAL ORIGIN OF CHINESE DONKEY BREEDS |
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| WANC Quan-xi , CHEN Jian-xing , MIN Ling-jiang , Wuritunasheng , Wu yun.GENETIC CIVERSITH AND MATERNAL ORIGIN OF CHINESE DONKEY BREEDS[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2012,33(2):7-11. | |
| Authors: | WANC Quan-xi CHEN Jian-xing MIN Ling-jiang Wuritunasheng Wu yun |
| Institution: | 1.Graduate Department,Inner Mongolia Agricultural University,Hohhot 010018,China; 2.College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China; 3.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China; 4.Alxa League Left Banner Barunbieli Animal Health Supervision Station,Alxa Left Banner 750300,China; 5.Agriculture and Animal Husbandry Bureau of Linxi County,Chifeng 025250,China) |
| Abstract: | To provide molecular biological foundation for the evaluation,conservation and utilization of Chinese domestic donkey genetic resources,we investigated the genetic diversity and maternal origin of domestic donkeys by detecting the variations of 308bp fragment of mtDNA Cytb gene sequence in Chinese donkey samples with self designed primers,using PCR-SSCP technique.Three genotypes were found by this way,and 15 variable sites were revealed and 17 haplotypes were determined after alignment with all the homologous 308nt Chinese donkey Cytb sequences deposited in GenBank.The results showed that abundant genetic diversity was revealed in the researched region of donkey Cytb,by calculating the genetic diversity indexes of all Chinese donkey populations.Additionally,it was demonstrated that the maternal origins of domestic donkeys are Nubian and Somali asses,two kinds of African wild asses,by comparison and Network analyses with the Cytb sequences of two different kinds of African wild asses. |
| Keywords: | Donkey Cytb Genetic Diversity Maternal Origin |
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