| 银与光所产生的协同效应的微生物灭活现象观察 |
| |
| 引用本文： | 金凤,何金山,宝音娜.银与光所产生的协同效应的微生物灭活现象观察[J].内蒙古农业大学学报(自然科学版),2012,33(2):12-15. |
| |
| 作者姓名： | 金凤 何金山 宝音娜 |
| |
| 作者单位： | 1. 内蒙古农业大学动物科学学院,呼和浩特,010018
2. 内蒙古大学蒙药化学研究所,呼和浩特,010018 |
| |
| 基金项目： | 内蒙古大学高层人才引进科研启动项目 |
| |
| 摘 要： | 研究发现在使用紫外线(UV-A,395nm)进行照射时,银溶液对微生物的灭活作用得到增强,特别是对真核微生物的灭活作用得到显著增强。为解明这种银与光所产生的协同效应的微生物灭活机理,使用电子自旋共振仪(Electron Spin Resonance,ESR)对溶液进行检测,并采用扫描电子显微镜(SEM)以及测定线粒体酶活性等方法,推测出了其作用机理,即在光照下氧化银(Ag2O)被激活并与水分子发生反应产生羟基自由基(.OH)。羟基自由基破坏真核微生物的细胞壁,失活其细胞内线粒体酶活性,从而引起真核微生物细胞死灭。
|
| 关 键 词： | 氧化银 紫外线 协同效应 真核微生物 |
INACTIVATION MECHANISM OF MICROORGANISMS BY THE SYNERGY OF SILVER AND LIGHT IRRADIATION |
| |
| JIN Fen
,
HE Jin-shan
,
Baoyina.INACTIVATION MECHANISM OF MICROORGANISMS BY THE SYNERGY OF SILVER AND LIGHT IRRADIATION[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2012,33(2):12-15. |
| |
| Authors: | JIN Fen HE Jin-shan Baoyina |
| |
| Institution: | JIN Fen1,HE Jin-shan2*,Baoyina1(1.College of Animal Science,Inner Mongolia Agricaltural University,Hohhot 010018,China; 2.Institute of Mongolian Medicine Chemistry,Inner Mongolia University.Hohhot,P.R.,China 010021) |
| |
| Abstract: | The inactivation efficiencies of microorganisms were found to be enhanced by using silver solution together with ultraviolet light(UV-A,395 nm) irradiation.The inactivation efficiencies were improved remarkably especially in eukaryotic microorganism.To make clear the inactivation mechanism of microorganisms by the combination effect of silver and ultraviolet light irradiation,the resultant solution was characterized by ESR(Electron Spin Resonance,ESR).Scanning electron microscopy(SEM) and the method for measuring enzyme activity of mitochondria for eukaryotic cells were used to conjecture the mechanism,It is proposed that silver oxide(Ag2O) can be activated by ultraviolet light irradiation and react with water molecules to produce hydroxyl radical(oOH).Hydroxyl radical could damage cell wall of eukaryotic microorganisms,and inactivate the enzyme activity of mitochondria of eukaryotic microorganism cells.Accordingly,eukaryotic microorganism cells would die. |
| |
| Keywords: | Silver oxide ultraviolet light synergistic effect hydroxyl radical microorganisms |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
