| 胡杨(Populus euphratica)盐相关基因的克隆 |
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| 引用本文： | 曾会明,沈昕.胡杨(Populus euphratica)盐相关基因的克隆[J].内蒙古农业大学学报(自然科学版),2004,25(2):76-80. |
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| 作者姓名： | 曾会明 沈昕 |
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| 作者单位： | 北京林业大学生物学院,北京,100083 |
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| 基金项目： | 国家自然科学基金 ( 30 170 76 3) |
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| 摘 要： | 以2年生胡杨实生苗为研究材料，通过用119mmol／L的NaCl溶液浇灌处理1h，分别收集NaCl处理前后的细嫩白根设立对照样品与处理样品，分离rnRNA。用抑制性扣除杂交技术建立胡杨盐处理的cDNA扣除文库，并以cDNA阵列杂交技术用。。P标记探针进行杂交，共筛选672个有效克隆，从中确认3个探针进行了序列分析，其中1个为盐抑制表达基因片段，2个为盐诱导表达基因片段。分析表明，1个盐诱导表达基因片段为1含188氨基酸开放阅读框的EST，该EST具有1079个核苷酸。该结果与我们早先检测到的1个胡杨盐诱导表达蛋白大小相符。
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| 关 键 词： | 胡杨 耐盐性 基因克隆 抑制性扣除杂交技术 序列分析 |
| 文章编号： | 1009-3575(2004)02-0076-05 |
| 修稿时间： | 2004年3月9日 |
SCREENING SALT-RELATED GENE IN POPULUS EUPHRATICA |
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| ZENG Hui-ming,SHEN Xin.SCREENING SALT-RELATED GENE IN POPULUS EUPHRATICA[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2004,25(2):76-80. |
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| Authors: | ZENG Hui-ming SHEN Xin |
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| Institution: | ZENG Hui-ming1,SHEN Xin |
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| Abstract: | Collected 2 year old Populus euphratica tender roots before and 1h after watered 119mmol/L NaCl. Extracted its mRNA to construct salt-related cDNA subtractive library by suppressed subtractive hybridization. Through hybridized with probes labeled ~(32)P, 672 clones had been screened and confirmed 1 of them was salt-suppressed gene segment and 2 were salt-inducible. Analysis shows a salt-suppressed segment is EST contained 188 amino acids opening reading frame, this result is in accordance with our earlier study on P. euphratica salt-inducible proteins. The salt-inducible gene EST is bankited to GenBank with accession number AF315118. |
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| Keywords: | Populus euphratica Salt-related gene Suppressed subtractive hybridization |
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