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用花粉管通道法将抗旱耐盐基因导入玉米自交系的研究
引用本文:董春林,张明义,林忠平,张彦芹,杨丽莉,梁改梅,孙洁.用花粉管通道法将抗旱耐盐基因导入玉米自交系的研究[J].山西农业科学,2011,39(5):392-394.
作者姓名:董春林  张明义  林忠平  张彦芹  杨丽莉  梁改梅  孙洁
作者单位:1. 山西省农业科学院旱地农业研究中心,山西,太原,030006
2. 北京大学生命科学学院,北京,100871
基金项目:国家转基因生物新品种培育重大专项
摘    要:采用改进的花粉管通道法,将海藻糖合酶基因(TPS)导入玉米自交系郑58中。3~5叶期时喷雾Basta(0.7%)溶液筛选,进行PCR检测,结果表明,目的基因已导入转化植株并整合到植株染色体上。田间初步抗旱性检测显示,转化植株(株系)的抗旱性高于对照植株。应用改进的花粉管通道法导入抗旱目的基因,可以得到玉米抗旱新资源,是一种切实有效的抗旱育种新方法。

关 键 词:玉米  花粉管通道技术  海藻糖合酶基因(TPS)  转基因

Study on Transferring Gene of Salt Tolerance and Drought Resistance into Corn Inbred Line by Pollen Tube Path
DONG Chun-lin,ZHANG Ming-yi,LIN Zhong-ping,ZHANG Yan-qin,YANG Li-li,LIANG Gai-mei,SUN Jie.Study on Transferring Gene of Salt Tolerance and Drought Resistance into Corn Inbred Line by Pollen Tube Path[J].Journal of Shanxi Agricultural Sciences,2011,39(5):392-394.
Authors:DONG Chun-lin  ZHANG Ming-yi  LIN Zhong-ping  ZHANG Yan-qin  YANG Li-li  LIANG Gai-mei  SUN Jie
Institution:DONG Chun-lin1,ZHANG Ming-yi1,LIN Zhong-ping2,ZHANG Yan-qin1,YANG Li-li1,LIANG Gai-mei1,SUN Jie1(1.Research Center of Arid Farming,Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China,2.College of Life Sciences,Beijing University,Beijing 100871,China)
Abstract:Using the improved pollen tube path method, trehalose synthase gene (TPS) was transferred into corn inbred line Zheng 58. In the 3 - 5 leaf stage, the selection of spraying Basta (0.7%)solution was combined with PCR test, the new gene had been imported into the plants and integrated into the plant chromosomes. The initial drought-tolerance investigation in fields con firmed that the drought-tolerance of transgenie plants was higher than the non-transformed. The results showed that the new corn drought resistance germplasm could be gained by pollen tube path, and it is a new feasible and effective way for corn drought resistance breeding.
Keywords:corn  pollen tube path  trehalose synthase gene  transgenic  
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