| 日本薯蓣脱毒培养及离体高效快繁体系的建立 |
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| 引用本文： | 解晓红,陈丽,裴蕾,李江辉,李波,解红娥,武宗信.日本薯蓣脱毒培养及离体高效快繁体系的建立[J].山西农业科学,2011,39(6):508-511. |
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| 作者姓名： | 解晓红 陈丽 裴蕾 李江辉 李波 解红娥 武宗信 |
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| 作者单位： | 山西省农业科学院棉花研究所,山西运城,044000 |
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| 摘 要： | 通过组织培养手段对日本薯蓣进行脱毒及快繁技术研究。结果表明:0.1%HgCl2消毒8 min效果最好,茎段成活率达88%;在MS培养基中,0.5 mg/L 6-BA和1.5 mg/L NAA配合使用时日本薯蓣茎尖的成活率高达100%且生长状况良好;MS+1.5 mg/L6-BA+0.5 mg/LNAA对茎段芽增殖的促进作用明显;MS+2.0mg/L KT+0.02 mg/L NAA可很好地诱导生根和促进生长,再生植株移栽成活率可达85.5%以上。
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| 关 键 词： | 日本薯蓣 脱毒 快繁 组织培养 茎尖培养 试管苗 |
Establishment of Virus-free and Rapid Propagation System of Rhizoma Dioscoreae |
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| XIE Xiao-hong,CHEN Li,PEI Lei,LI Jiang-hui,LI Bo,XIE Hong-e,WU Zong-xin.Establishment of Virus-free and Rapid Propagation System of Rhizoma Dioscoreae[J].Journal of Shanxi Agricultural Sciences,2011,39(6):508-511. |
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| Authors: | XIE Xiao-hong CHEN Li PEI Lei LI Jiang-hui LI Bo XIE Hong-e WU Zong-xin |
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| Institution: | XIE Xiao-hong,CHEN Li,PEI Lei,LI Jiang-hui,LI Bo,XIE Hong-e,WU Zong-xin(Institute of Cotton,Shanxi Academy of Agricultural Sciences,Yuncheng 044000,China) |
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| Abstract: | Virus-free and rapid propagation system of Rhizoma Dioscoreae was established via shoot-tip culture.The results showed: Shoot apices rinsing for 8 min in 0.1% HgCl2 could obtained the best disinfection and survival rate up to 88%;Shoot-tip from aseptic seedling inoculated in MS+0.5 mg/L 6-BA+1.5 mg/L NAA had the best growth conditions and the survival rate reached 100%;MS+1.5 mg/L 6-BA+0.5 mg/L NAA shoot proliferation of the stem fragments of virus-free was promoted obviously;root system was well induced an... |
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| Keywords: | Rhizoma Dioscoreae virus-free rapid propagation tissue culture stem tip culture test-tube plantlet |
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